Multielemental Analysis of Low-Volume Samples Reveals Cancer-Specific Profile in Serum and Sorted Immune Cells
| Autor: | Serge Rezzi, Alessio Palini, Mukul Girotra, Nicola Vannini, Julie Laval, Gabriele Dammone, Jean-Philippe Godin, Tobias Konz, Marcela Rincon Restrepo, George Coukos, Federico Sizzano, Caroline Monnard |
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| Rok vydání: | 2020 |
| Předmět: |
Oncology
medicine.medical_specialty T-Lymphocytes Sample (material) 010402 general chemistry 01 natural sciences Mass Spectrometry Analytical Chemistry Mice Immune system Limit of Detection Cell Line Tumor Neoplasms Internal medicine medicine Animals Transplantation Homologous Magnesium Chemistry 010401 analytical chemistry Cancer medicine.disease 0104 chemical sciences Mice Inbred C57BL Low volume Zinc Inorganic Chemicals Copper |
| Zdroj: | Analytical Chemistry. 92:8750-8758 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.9b05643 |
| Popis: | We developed and validated a reliable, robust, and easy-to-implement quantitative method for multielemental analysis of low-volume samples. Our ICP-MS-based method comprises the analysis of 20 elements (Mg, P, S, K, Ca, V, Cr, Mn, Fe, Co, Cu, Zn, Se, Br, Rb, Sr, Mo, I, Cs, and Ba) in 10 μL of serum and 12 elements (Mg, S, Mn, Fe, Co, Cu, Zn Se, Br, Rb, Mo, and Cs) in less than 250 000 cells. As a proof-of-concept, we analyzed the elemental profiles of serum and sorted immune T cells derived from naı̈ve and tumor-bearing mice. The results indicate a tumor systemic effect on the elemental profiles of both serum and T cells. Our approach highlights promising applications of multielemental analysis in precious samples such as rare cell populations or limited volumes of biofluids that could provide a deeper understanding of the essential role of elements as cofactors in biological and pathological processes. |
| Databáze: | OpenAIRE |
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