Effect of the proton motive force inhibitor carbonyl cyanide-m-chlorophenylhydrazone (CCCP) on Pseudomonas aeruginosa biofilm development
| Autor: | Antonios N. Maniatis, Alexandros Ikonomidis, Athanassios Tsakris, Spyros Pournaras, M. Kanellopoulou |
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| Rok vydání: | 2009 |
| Předmět: |
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Strain (chemistry) Pseudomonas aeruginosa Uncoupling Agents Biofilm Proton-Motive Force Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition Biology biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Bacterial Proteins Biofilms Pseudomonadales medicine Humans Autoinducer Efflux Bacteria Pseudomonadaceae |
| Zdroj: | Letters in applied microbiology. 47(4) |
| ISSN: | 0266-8254 |
| Popis: | Aims: Proton motive force (PMF) inhibition enhances the intracellular accumulation of autoinducers possibly interfering with biofilm formation. We evaluated the effect of the PMF inhibitor carbonyl cyanide-m-chlorophenylhydrazone (CCCP) on Pseudomonas aeruginosa biofilm development. Methods and Results: Four epidemiologically unrelated P. aeruginosa isolates were studied. A MexAB-oprM overproducing strain was used as control. Expression of gene mexB was examined and biofilm formation after incubation with 0, 12·5 and 25 μmol l−1 of CCCP was investigated. Mean values of optical density were analysed with one-way analysis of variance and t-test. Two isolates subexpressed mexB gene and only 25 μmol l−1 of CCCP affected biofilm formation. Biofilms of the other two isolates and control strain PA140 exhibited significantly lower absorbance (P ranging from |
| Databáze: | OpenAIRE |
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