Development of a dendrimer PAMAM‑based gold biochip for rapid and sensitive detection of endogenous IFN‑γ and anti‑IFN‑γ IgG in patients with hemophagocytic lymphohistiocytosis
| Autor: | Hui Lv, Yi Gao, Yi‑Xin Du, Qian Liu, Marion Schneider, Sheng‑Sheng Liu, Lei Ye, Zi‑Jian Song, Song‑Guo Li, Wei‑Dong Du, Jian Hong |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Cancer Research dendrimer Biochemistry 0302 clinical medicine Interferon Child Biochip biology Chemistry Articles polyamidoamine Middle Aged Oncology Child Preschool 030220 oncology & carcinogenesis Cytokines Molecular Medicine Female Antibody medicine.drug Adult China Dendrimers Adolescent Protein Array Analysis protein biochip Lymphohistiocytosis Hemophagocytic Interferon-gamma 03 medical and health sciences Immune system interferon-γ Genetics medicine Humans Molecular Biology Hemophagocytic lymphohistiocytosis Infant Newborn Infant medicine.disease Molecular medicine Molecular biology 030104 developmental biology hemophagocytic lymphohistiocytosis Apoptosis Immunoglobulin G biology.protein Gold Cytokine storm |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2020.11605 |
| Popis: | Hemophagocytic lymphohistiocytosis (HLH) is a rare but severe disease characterized by immune hyperactivation and cytokine storm. Given the high mortality rate of HLH, there is a need for more effective diagnostic tools and treatments. The present study developed a dendrimer‑based protein biochip for rapid, sensitive and simultaneous detection of serum interferon (IFN)‑γ and endogenous anti‑IFN‑γ antibody (Ab) in patients with HLH. A gold biochip was modified with 1, 4‑phenylene diisothiocyanate (PDITC), polyamidoamine (PAMAM) or PDITC‑activated PAMAM. The optimal immobilization concentration for Ab capture and the reaction concentration for detecting Ab on the PDITC‑activated PAMAM‑modified biochip were 6.25 and 3.12 µg/ml, respectively; the limit of detection of IFN‑γ protein was 50 pg/ml. The efficiency of the protein‑probed biochip in detecting IFN‑γ and anti‑IFN‑γ Ab in serum samples from 77 patients with HLH was evaluated; the positive rates for IFN‑γ and anti‑IFN‑γ IgG Ab were 63.6% (49/77) and 61.0% (47/77), respectively. The present results demonstrated that the PDITC‑activated PAMAM‑modified biochip might be a sensitive tool for the specific detection of IFN‑γ and anti‑IFN‑γ Ab in serum, and might have clinical applicability for the diagnosis of HLH. |
| Databáze: | OpenAIRE |
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