Lipopolysaccharides and Beta-Glucuronidase Activity in Choledochal Bile in Relation to Choledocholithiasis
| Autor: | Olav Sandstad, V Skar, M Osnes, Terje Osnes |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Gram-negative bacteria Lipopolysaccharide Gallstones Gastroenterology Microbiology Pathogenesis chemistry.chemical_compound Internal medicine Gram-Negative Bacteria medicine Bile Humans Duodenal Diseases Limulus Test Aged Glucuronidase Endoscopic retrograde cholangiopancreatography biology medicine.diagnostic_test Beta-glucuronidase activity Biological activity biology.organism_classification medicine.disease Pathophysiology Diverticulum Logistic Models chemistry Female |
| Zdroj: | Digestion. 58:437-443 |
| ISSN: | 1421-9867 0012-2823 |
| Popis: | Common duct gallstones are mainly of the brown pigment type, which are usually attributed to bacterial factors. Bacterial beta-glucuronidase most probably plays a role in the pathogenesis in many but not all patients. The role of other bacterial factors is more undecided. The aims of this study were to investigate a possible association between lipopolysaccharides (LPS) and choledocholithiasis, and to examine the interrelationship to beta-glucuronidase. Common duct bile obtained at endoscopic retrograde cholangiography in 86 patients was assayed for LPS by a limulus amebocyte lysate test, and beta-glucuronidase activity at pH 7.0 was measured. We found that both elevated concentration of LPS and the presence of juxtapapillary duodenal diverticula were associated with common duct stones (p0.01, both). Patients who had their common duct stones removed recently had a lower LPS concentration and a lower activity of beta-glucuronidase than those who had a stone in situ (p0.01, both), but still higher LPS concentration than those without choledocholithiasis at all (p0.01). In multiple logistic regression analysis, elevated LPS was the significant predictor of common duct stones (p0.01), and not confounding with neither beta-glucuronidase nor juxtapapillary diverticula. We conclude that gram-negative bacteria convey bacterial factors associated with choledocholithiasis, by mechanisms independent of, and additional to beta-glucuronidase. |
| Databáze: | OpenAIRE |
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