Interneuronal exchange and functional integration of synaptobrevin via extracellular vesicles
| Autor: | Aldo Alejandro Vilcaes, Ege T. Kavalali, Natali L. Chanaday |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Cell type Vesicle-Associated Membrane Protein 2 Synaptobrevin Neurotransmission Inhibitory postsynaptic potential Synaptic Transmission Synaptic vesicle Article Rats Sprague-Dawley Extracellular Vesicles Mice 03 medical and health sciences 0302 clinical medicine Extracellular Animals Mice Knockout Neurons Chemistry General Neuroscience Vesicle Synaptic vesicle cycle Rats Cell biology 030104 developmental biology Female 030217 neurology & neurosurgery |
| Zdroj: | Neuron |
| ISSN: | 0896-6273 |
| Popis: | Recent studies have investigated the composition and functional effects of extracellular vesicles (EVs) secreted by a variety of cell types. However, the mechanisms underlying the impact of these vesicles on neurotransmission remain unclear. Here, we isolated EVs secreted by rat and mouse hippocampal neurons and found that they contain synaptic-vesicle-associated proteins, in particular the vesicular SNARE (soluble N-ethylmaleimide-sensitive factor [NSF]-attachment protein receptor) synaptobrevin (also called VAMP). Using a combination of electrophysiology and live-fluorescence imaging, we demonstrate that this extracellular pool of synaptobrevins can rapidly integrate into the synaptic vesicle cycle of host neurons via a CD81-dependent process and selectively augment inhibitory neurotransmission as well as specifically rescue neurotransmission in synapses deficient in synaptobrevin. These findings uncover a novel means of interneuronal communication and functional coupling via exchange of vesicular SNAREs. |
| Databáze: | OpenAIRE |
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