Multiarray on a test strip (MATS): rapid multiplex immunodetection of priority potato pathogens
| Autor: | Anatoly V. Zherdev, Irina V. Safenkova, Yuri A. Varitsev, Ilya A. Zaitsev, Galina Pankratova, Boris B. Dzantiev, Y. Y. Vengerov |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Protein Array Analysis Metal Nanoparticles 01 natural sciences Biochemistry Chromatography Affinity Virus Plant Viruses Analytical Chemistry 03 medical and health sciences Limit of Detection Multiplex Potato virus A Plant Diseases Reagent Strips Solanum tuberosum Chromatography Spots biology fungi 010401 analytical chemistry Potato virus M food and beverages Equipment Design biology.organism_classification Potato virus X 0104 chemical sciences 030104 developmental biology Potato virus Y Gold Antibodies Immobilized Clavibacter michiganensis |
| Zdroj: | Analytical and Bioanalytical Chemistry. 408:6009-6017 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-016-9463-6 |
| Popis: | Multiarray on a test strip (MATS) was developed for the detection of eight important potato pathogens. The proposed assay combines the rapidity of immunochromatography with the high throughput of array techniques. The test zone of the immunochromatographic strip comprises ordered rows of spots containing antibodies specific for different potato pathogens. The assay benefits from the simplicity of immunochromatography; colored immune complexes form at the corresponding spots within the test zone. The presence and intensity of the coloration are used for identification of the target pathogens. The MATS was applied to the simultaneous detection of eight priority potato pathogens, characterized by the following limits of detection: 1 ng/mL for potato virus X and the ordinary type of potato virus Y, 10 ng/mL for potato virus M, 20 ng/mL for potato leaf roll virus, 40 ng/mL for necrotic-type potato virus Y, 100 ng/mL for potato virus S, 300 ng/mL for potato virus A, and 10(4) cells/mL for Clavibacter michiganensis subsp. sepedonicus. Analysis time was 15 min. The observed sensitivity of the MATS was comparable to the traditional enzyme-linked immunosorbent assay. The developed technique was tested on potato leaf extracts, and its efficiency for on-site control of the pathogens was confirmed in 100 % by commercial LFIA test strips. Graphical abstract Location of binding zones in the developed multiarray on a test strip (MATS) for simultaneous detection of eight pathogens. |
| Databáze: | OpenAIRE |
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