Association of changes in ER stress-mediated signaling pathway with lead-induced insulin resistance and apoptosis in rats and their prevention by A-type dimeric epigallocatechin-3-gallate
| Autor: | Zhao-Jun Feng, Wei Yang, Chao Cheng, Hong Jiang, Jie-Qiong Ma, Chan-Min Liu, Jian-Mei Sun |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine medicine.medical_treatment Apoptosis Toxicology medicine.disease_cause Catechin 0302 clinical medicine Insulin heterocyclic compounds Endoplasmic Reticulum Chaperone BiP biology Cytochrome c Cytochromes c food and beverages General Medicine Endoplasmic Reticulum Stress Liver 030220 oncology & carcinogenesis Dimerization Signal Transduction medicine.drug medicine.medical_specialty complex mixtures 03 medical and health sciences Insulin resistance Metabolic Diseases Internal medicine medicine Animals Humans Plant Extracts Endoplasmic reticulum Diospyros medicine.disease Rats Oxidative Stress 030104 developmental biology Endocrinology Lead biology.protein Unfolded protein response sense organs Insulin Resistance Pioglitazone Oxidative stress Food Science |
| Zdroj: | Food and Chemical Toxicology. 110:325-332 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2017.10.040 |
| Popis: | A-type dimeric epigallocatechin-3-gallate (A-type-EGCG-dimer, AEd), a new proanthocyanidins dimer from persimmon fruits, has been shown to have health benefit effects. However, A-type-EGCG-dimer affects gluose metabolism in the liver and the underlying mechanism is not clarified. The present study aims to examine the protective effects of A-type-EGCG-dimer on Pb-induced hepatic insulin resistance, endoplasmic reticulum (ER) stress and apoptosis in rats. Male wistar rats exposed to 0.05% w/v Pb acetate in the drinking water with or without A-type-EGCG-dimer coadministration (200 mg/kg body weight/day, intragastrically) for three months. We found that A-type-EGCG-dimer and pioglitazone supplementation significantly deceased glucose and insulin levels in plasma as compared with the Pb group. A-type-EGCG-dimer markedly prevents Pb-induced oxidative stress, ER stress and apoptosis in livers. A-type-EGCG-dimer and pioglitazone reduced the expression levels of the GRP78, PEPCK, G6Pase, p-PERK, p-IRE1, p-JNK, ATF4, CHOP and increased p-AKT in livers of the Pb group. Moreover, A-type-EGCG-dimer reduced ROS production and restored the activities of SOD and GPx in livers. A-type-EGCG-dimer decreased Bax, cytosolic cytochrome c and cleaved caspase-3 and increased Bcl-2 in livers of Pb-exposed rats. Our results suggest that A-type-EGCG-dimer might be a potential natural candidate for the prevention of hepatic insulin resistance and apoptosis induced by Pb. |
| Databáze: | OpenAIRE |
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