Utility of NKX3.1 immunohistochemistry in the differential diagnosis of seminal vesicles versus prostatic tissue in needle biopsy
| Autor: | Kristyna Pivovarcikova, Milan Hora, Tomáš Pitra, Joanna Rogala, Reza Alaghehbandan, Ondrej Hes, Eva Maria Comperat, Maryna Slisarenko, Michal Michal |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Adenocarcinoma Pathology and Forensic Medicine Diagnosis Differential 03 medical and health sciences 0302 clinical medicine Prostate Biomarkers Tumor Medicine Humans Aged Submucosal glands Aged 80 and over Homeodomain Proteins biology urogenital system business.industry Vesicle Biopsy Needle Prostatic Neoplasms Seminal Vesicles General Medicine Ductal carcinoma Middle Aged medicine.disease Immunohistochemistry Mesenchymal chondrosarcoma 030104 developmental biology medicine.anatomical_structure Polyclonal antibodies 030220 oncology & carcinogenesis biology.protein business Rete Testis Adenocarcinoma Transcription Factors |
| Zdroj: | Annals of diagnostic pathology. 49 |
| ISSN: | 1532-8198 |
| Popis: | NKX3.1 is considered a reliable immunohistochemical marker of prostatic origin with high specificity and sensitivity. However, NKX3.1 positivity has been described in other neoplastic and non-neoplastic tissues, such as mesenchymal chondrosarcoma, sex-cord stromal tumors, rete testis adenocarcinoma, lobular and ductal carcinoma of the breast, salivary glands, peribronchial submucosal glands, and Sertoli cells. We analyzed expression of two antibodies (mono and polyclonal) of NKX3.1 in a total of 63 non-neoplastic seminal vesicles. We used 52 resection materials (12 seminal vesicles without prostatic adenocarcinoma, 26 seminal vesicles with prostatic adenocarcinoma infiltration, and 14 cases of seminal vesicles infiltrated by urothelial carcinoma) and 11 prostatic core needle biopsies with incidentally sampled fragment of seminal vesicles. In all cases, tissues from seminal vesicles were completely negative for NKX3.1, despite using polyclonal and monoclonal NKX3.1 antibodies, and regardless of the detection system utilized (diaminobenzidine (DAB) versus alkaline phosphatase (AF)). However, prostatic adenocarcinoma was negative in several cases (n = 6), when AF detection system was used. Reaction with DAB was strong and robust in all cases. Based on our data, we can recommend NKX3.1 as a negative immunohistochemical marker of seminal vesicles. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect