A new era for functional labeling of neurons: activity-dependent promoters have come of age
| Autor: | Takashi eKawashima, Hiroyuki eOkuno, Haruhiko eBito |
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| Předmět: |
c-fos
Cognitive Neuroscience Neuroscience (miscellaneous) Review Article Biology c-Fos lcsh:RC321-571 Cellular and Molecular Neuroscience Transcription (biology) Live cell imaging Animals activity-dependent gene expression Promoter Regions Genetic Genes Immediate-Early lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Enhancer Elements Neurons E-SARE Repertoire Promoter live imaging Arc Sensory Systems biology.protein neuronal ensemble Immediate early gene Neuroscience |
| Zdroj: | Scopus-Elsevier Frontiers in Neural Circuits, Vol 8 (2014) Frontiers in Neural Circuits |
| Popis: | Genetic labeling of neurons with a specific response feature is an emerging technology for precise dissection of brain circuits that are functionally heterogeneous at the single-cell level. While immediate early gene mapping has been widely used for decades to identify brain regions which are activated by external stimuli, recent characterization of the promoter and enhancer elements responsible for neuronal activity-dependent transcription have opened new avenues for live imaging of active neurons. Indeed, these advancements provided the basis for a growing repertoire of novel experiments to address the role of active neuronal networks in cognitive behaviors. In this review, we summarize the current literature on the usage and development of activity-dependent promoters and discuss the future directions of this expanding new field. |
| Databáze: | OpenAIRE |
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