Human Desmocollin 1 (Dsc1) Is an Autoantigen for the Subcorneal Pustular Dermatosis Type of IgA Pemphigus
| Autor: | Masayuki Amagai, Martyn A.J. Chidgey, Osamu Mori, Takeji Nishikawa, Chie Kiyokawa, David R. Garrod, Koji Komori, Ken Ishii, Yasushi Kobayashi, Takashi Hashimoto, Minoru Miyasato |
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| Rok vydání: | 1997 |
| Předmět: |
DNA
Complementary Immunoblotting keratinocyte Dermatology Biology Desmoglein Autoantigens Biochemistry Antigen Desmosome medicine Animals Humans IgA pemphigus mammalian cell transfection Cloning Molecular Pemphigus herpetiformis Molecular Biology Skin Desmocollins DSC3 Membrane Glycoproteins integumentary system Skin Diseases Vesiculobullous Desmosomes Cell Biology medicine.disease Immunoglobulin A Pemphigus medicine.anatomical_structure Fluorescent Antibody Technique Direct Immunology autoimmune bullous disease COS Cells Desmocollin desmosome |
| Zdroj: | Journal of Investigative Dermatology. 109(2):127-131 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/1523-1747.ep12319025 |
| Popis: | IgA pemphigus showing IgA anti-keratinocyte cell surface autoantibodies is divided into subcorneal pustular dermatosis (SPD) and intraepidermal neutrophilic IgA dermatosis (IEN) types. We previously showed by immunoblotting that IgA from some IgA pemphigus patients reacted with bovine desmocollins (Dsc), but not human Dsc. To determine the antigen for IgA pemphigus, we focused on conformation-dependent epitopes of Dsc, because sera of patients with classical pemphigus recognize conformation-sensitive epitopes of desmogleins. We constructed mammalian expression vectors containing the entire coding sequences of human Dsc1, Dsc2, and Dsc3 and transiently transfected them into COS7 cells by lipofection. Immunofluorescence of COS7 cells transfected with single human Dscs showed that IgA antibodies of all six SPD-type IgA pemphigus cases reacted with the surface of cells expressing Dsc1, but not with cells expressing Dsc2 or Dsc3. In contrast, none of seven IEN-type IgA pemphigus cases reacted with cells transfected with any Dscs. These results convincingly indicate that human Dsc1 is an autoantigen for SPD-type IgA pemphigus, suggesting the possibility of an important role for Dsc1 in the pathogenesis of this disease. This study shows that a Dsc can be an autoimmune target in human skin disease. |
| Databáze: | OpenAIRE |
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