| Autor: |
J.H. Yao, H. B. Tian, Shimin Liu, Chen Zheng, Long Guo, Yaoquan Liu, Y.C. Cao, Xiurong Xu, Chuanjiang Cai |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Animal, Vol 13, Iss 9, Pp 1899-1906 (2019) |
| ISSN: |
1751-7311 |
| DOI: |
10.1017/s1751731118003683 |
| Popis: |
Starch digestion in the small intestines of the dairy cow is low, to a large extent, due to a shortage of syntheses of α-amylase. One strategy to improve the situation is to enhance the synthesis of α-amylase. The mammalian target of rapamycin (mTOR) signalling pathway, which acts as a central regulator of protein synthesis, can be activated by leucine. Our objectives were to investigate the effects of leucine on the mTOR signalling pathway and to define the associations between these signalling activities and the synthesis of pancreatic enzymes using an in vitro model of cultured Holstein dairy calf pancreatic tissue. The pancreatic tissue was incubated in culture medium containing l-leucine for 3 h, and samples were collected hourly, with the control being included but not containing l-leucine. The leucine supplementation increased α-amylase and trypsin activities and the messenger RNA expression of their coding genes (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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