A Salmonella enterica Serovar Typhimurium Succinate Dehydrogenase/Fumarate Reductase Double Mutant Is Avirulent and Immunogenic in BALB/c Mice
| Autor: | Mary P. Leatham, Tyrrell Conway, Eric J. Gauger, Regino Mercado-Lubo, Paul S. Cohen |
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| Rok vydání: | 2008 |
| Předmět: |
Salmonella typhimurium
Immunology Mutant Colony Count Microbial Virulence Microbiology BALB/c Mice Peyer's Patches Animals Mice Inbred BALB C Salmonella Infections Animal biology Succinate dehydrogenase Bacterial Infections Fumarate reductase biology.organism_classification Survival Analysis Enterobacteriaceae Succinate Dehydrogenase Citric acid cycle Infectious Diseases Liver Salmonella enterica biology.protein Female Parasitology Gene Deletion Spleen |
| Zdroj: | Infection and Immunity. 76:1128-1134 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.01226-07 |
| Popis: | Previously we showed that the tricarboxylic acid (TCA) cycle operates as a full cycle during Salmonella enterica serovar Typhimurium SR-11 peroral infection of BALB/c mice (M. Tchawa Yimga et al., Infect. Immun. 74:1130-1140, 2006). The evidence was that a Δ sucCD mutant (succinyl coenzyme A [succinyl-CoA] synthetase), which prevents the conversion of succinyl-CoA to succinate, and a Δ sdhCDA mutant (succinate dehydrogenase), which blocks the conversion of succinate to fumarate, were both attenuated, whereas an SR-11 Δ aspA mutant (aspartase) and an SR-11 Δ frdABCD mutant (fumarate reductase), deficient in the ability to run the reductive branch of the TCA cycle, were fully virulent. In the present study, evidence is presented that a serovar Typhimurium SR-11 Δ frdABCD Δ sdhCDA double mutant is avirulent in BALB/c mice and protective against subsequent infection with the virulent serovar Typhimurium SR-11 wild-type strain via the peroral route and is highly attenuated via the intraperitoneal route. These results suggest that fumarate reductase, which normally runs in the reductive pathway in the opposite direction of succinate dehydrogenase, can replace it during infection by running in the same direction as succinate dehydrogenase in order to run a full TCA cycle in an SR-11 Δ sdhCDA mutant. The data also suggest that the conversion of succinate to fumarate plays a key role in serovar Typhimurium virulence. Moreover, the data raise the possibility that S. enterica Δ frdABCD Δ sdhCDA double mutants and Δ frdABCD Δ sdhCDA double mutants of other intracellular bacterial pathogens with complete TCA cycles may prove to be effective live vaccine strains for animals and humans. |
| Databáze: | OpenAIRE |
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