Border Control—A Membrane-Linked Interactome of Arabidopsis
| Autor: | Zhao Su, Saman A. Parsa, Jin Chen, Rui-Sheng Wang, Erika Smith-Valle, Guido Grossmann, June M. Kwak, Tianying Su, Qunfeng Dong, Chang Hun You, Maria Sardi, Réjane Pratelli, Seung Y. Rhee, Biswa R. Acharya, Yuan Hu Xuan, Guillaume Pilot, Wolf B. Frommer, Sylvie Lalonde, Florent Villiers, Kouji Takeda, Chuanli Ju, Réka Albert, Alexander M. Jones, Julian I. Schroeder, Cheng-Hsun Ho, Meng Xu, Keith A. Frazer, Sarah M. Assmann, Heng-Cheng Hu |
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| Rok vydání: | 2014 |
| Předmět: |
Multidisciplinary
biology Arabidopsis Proteins Chemistry Cell Membrane Arabidopsis Membrane Proteins Aquaporin biology.organism_classification Bioinformatics Interactome Cell biology Potassium channel activity Membrane protein Two-Hybrid System Techniques Phosphorylation Protein Interaction Maps Signal transduction Heterotrimeric G Protein Subunit Signal Transduction |
| Zdroj: | Science. 344:711-716 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.1251358 |
| Popis: | Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 10(6) pairs, we identified 12,102 membrane/signaling protein interactions from Arabidopsis. Besides confirmation of expected interactions such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, >99% of the interactions were previously unknown. Interactions were confirmed at a rate of 32% in orthogonal in planta split-green flourescent protein interaction assays, which was statistically indistinguishable from the confirmation rate for known interactions collected from literature (38%). Regulatory associations in membrane protein trafficking, turnover, and phosphorylation include regulation of potassium channel activity through abscisic acid signaling, transporter activity by a WNK kinase, and a brassinolide receptor kinase by trafficking-related proteins. These examples underscore the utility of the membrane/signaling protein interaction network for gene discovery and hypothesis generation in plants and other organisms. |
| Databáze: | OpenAIRE |
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