Further characterization of estrogen receptors in the human oviduct
| Autor: | Eberhard K. Muechler, Donna Cary |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Estrogen receptor In Vitro Techniques Biology Binding Competitive Cytosol Internal medicine Centrifugation Density Gradient medicine Humans Estrogen binding Fallopian Tubes Cell Nucleus Danazol Obstetrics and Gynecology Dextrans Middle Aged Ligand (biochemistry) Antiestrogen Norethynodrel Endocrinology Receptors Estrogen Reproductive Medicine Estrogen Charcoal Oviduct Female hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Fertility and Sterility. 39:819-823 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(16)47123-2 |
| Popis: | Estrogen binding in human oviducts was studied in vitro by the dextran-coated charcoal assay and sucrose density ultracentrifugation. Estrogen binds with high affinity and limited capacity to cytosol of the human oviduct. The concentration of competitive inhibitors to produce 50% reduction in estrogen binding was 8 x 10(-8) M for the antiestrogen CI-628, 8 x 10(-7) M for the progestogen norethynodrel, and 3 x 10(-6) M for the testosterone derivative danazol at the ligand concentration of 1 nM estradiol. Nuclear estrogen binding was not inhibited by a 100-fold excess of progesterone or by a 10-fold excess of norethynodrel. Estrogen-binding protein with a sedimentation coefficient of 4S was seen in oviductal cytosol of all three anatomic segments. The nuclear 4S peak of estrogen binding was demonstrated in the ampullary tubal segment. |
| Databáze: | OpenAIRE |
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