| Autor: |
Duan, Ranran, Sun, Ke, Fang, Fang, Wang, Ning, He, Ruya, Gao, Yang, Jing, Lijun, Li, Yanfei, Gong, Zhe, Yao, Yaobing, Luan, Tingting, Zhang, Chaopeng, Zhang, Jinwei, Zhao, Yi, Xie, Haojie, Zhou, Yongyan, Teng, Junfang, Zhang, Jinfeng, Jia, Yanjie |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.20762799.v1 |
| Popis: |
Additional file 1: Figure S1. Transmission electron microscopy (TEM) image of the TP NPs at pH 7.4. Scale bar, 100 nm. Figure S2. Biological transmission electron microscopy (Bio-TEM) image of the TPC@M2 NPs at pH 7.4. Scale bar, 200 nm. Figure S3. The results of dynamic light scattering (DLS) of the TPC@M2 NPs after incubation at 37 ℃ in PBS for different times (n = 3). Results are presented as means ± s.d. *P < 0.05, **P < 0.01, and ***P < 0.001 determined by Student’s t test. Figure S4. The results of hemolytic experiment of the TPC NPs and TPC@M2 NPs after incubation at 37 ℃ in red cell suspension for 12 h. Figure S4. The results of hemolytic experiment of the TPC NPs and TPC@M2 NPs after incubation at 37℃ in red cell suspension for 12 h. Figure S5. The O2 concentration changes in H2O2 solutions (750 μM) after incubation of various concentrations of TPC@M2 NPs. Figure S6. The relative enzymatic activity changes of free catalase and TPC@M2 NPs after protease K digestion for different periods. Figure S7. Mean fluorescence intensity (MFI) of Fe2+ levels in BV-2 cells after different treatment (n = 3). Results are presented as means ± s.d. *P < 0.05, **P < 0.01, and ***P < 0.001 determined by Student’s t test. Figure S8. Representative flow cytometric analysis of the M2 biomarkers (CD206+) and (b) the M1 biomarkers (CD16/32+) on the BV-2 cells treated with different groups (n = 3). Figure S9. The cell viabilities of rat primary neuronal cells and PC-12 cells treated with different concentrations of the TPC@M2 NPs for 24 h (n = 6). Figure S10. Mean fluorescence intensity (MFI) of PC-12 cells uptaked with different treatments (n = 3). Results are presented as means ± s.d. *P < 0.05, **P < 0.01, and ***P < 0.001 determined by Student’s t test. Figure S11. Brain iron content at days 3 of MCAO rats in different treatment group (n = 5). Results are presented as means ± s.d. *P < 0.05, **P < 0.01, and ***P < 0.001 determined by Student’s t test. Figure S12. Major liver function indexes (A), kidney function indexes (B) and blood routine indexes (C) of MCAO rats in different treatment group (n = 5). Results are presented as means ± s.d. *P < 0.05, **P < 0.01, and ***P < 0.001 determined by Student’s t test. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
