Raman Spectroscopy of Fish Oil Capsules: Polyunsaturated Fatty Acid Quantitation Plus Detection of Ethyl Esters and Oxidation
Autor: | Daniel P. Killeen, Keith C. Gordon, Susan N. Marshall, Nigel B. Perry, Elaine J. Burgess |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
food.ingredient Capsules Spectrum Analysis Raman 01 natural sciences Gelatin Peroxide Ether 03 medical and health sciences chemistry.chemical_compound food Fish Oils medicine Fatty acid methyl ester chemistry.chemical_classification 030109 nutrition & dietetics Chromatography 010401 analytical chemistry food and beverages Fatty acid General Chemistry Fish oil 0104 chemical sciences chemistry Docosahexaenoic acid Dietary Supplements Fatty Acids Unsaturated General Agricultural and Biological Sciences Softgel Polyunsaturated fatty acid medicine.drug |
Zdroj: | Journal of agricultural and food chemistry. 65(17) |
ISSN: | 1520-5118 |
Popis: | Fish oils are the primary dietary source of ω-3 polyunsaturated fatty acids (PUFA), but these compounds are prone to oxidation, and commercial fish oil supplements sometimes contain less PUFA than claimed. These supplements are predominantly sold in softgel capsules. In this work, we show that Fourier transform (FT)–Raman spectra of fish oils (n = 5) and ω-3 PUFA concentrates (n = 6) can be acquired directly through intact softgel (gelatin) capsules. These spectra could be used to rapidly distinguish supplements containing ethyl esters from those containing triacylglyceride oils. Raman spectroscopy calibrated with partial least-squares regression against traditional fatty acid methyl ester analyses by gas chromatography–mass spectrometry could be used to rapidly and nondestructively quantitate PUFA and other fatty acid classes directly though capsules. We also show that FT–Raman spectroscopy can noninvasively detect oxidation with high sensitivity. Oils with peroxide values of as low as 10 mequiv kg–1, wh... |
Databáze: | OpenAIRE |
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