Decreased susceptibility to antifungals in respiratory-deficient Kluyveromyces lactis mutants
| Autor: | Yvetta Gbelska, K. Balková, Marcela Sarinova, V. Straková |
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| Rok vydání: | 2008 |
| Předmět: |
Antifungal Agents
Bifonazole Mutant Microbial Sensitivity Tests Microbiology Cell wall Electron Transport Fungal Proteins Kluyveromyces Cell Wall Drug Resistance Fungal Multienzyme Complexes Amphotericin B medicine Kluyveromyces lactis Oxidase test biology Strain (chemistry) Glucan Endo-1 3-beta-D-Glucosidase General Medicine biology.organism_classification Blotting Northern Flow Cytometry Sterol Mitochondria Sterols Mutation medicine.drug Peptide Hydrolases |
| Zdroj: | Folia microbiologica. 52(5) |
| ISSN: | 0015-5632 |
| Popis: | Decreased susceptibility of K. lactis mutants impaired in the function of cytochrome c, cytochrome c1 and cytochrome-c oxidase to fluconazole, bifonazole and amphotericin B in comparison with the isogenic wild-type strain was observed. Flow cytometry with rhodamine 6G did not show any changes in the accumulation of the dye in the mutant cells compared with the corresponding wild-type strain. Sterol analysis showed similar overall amount of sterols in both wild-type and mutant cells. Taking into account the increased amphotericin B resistance and significantly diminished susceptibility of mutant cells to lyticase digestion, the cell wall structure and/or composition may probably be responsible for the observed changes in the susceptibility of mutants to the antifungal compounds used. |
| Databáze: | OpenAIRE |
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