3D-poly (lactic acid) scaffolds coated with gelatin and mucic acid for bone tissue engineering
| Autor: | Shantanu Patil, Mariappanadar Vairamani, Balakrishnan Abinaya, S. Viji Chandran, L. Roshini Yadav, Nagarajan Selvamurugan, Badrinath Ashwin, T.P. Prasith |
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| Rok vydání: | 2020 |
| Předmět: |
food.ingredient
Polyesters Mucic acid 02 engineering and technology Biochemistry Gelatin Bone and Bones Mice 03 medical and health sciences chemistry.chemical_compound food Coated Materials Biocompatible Structural Biology medicine Animals Bone regeneration Molecular Biology 030304 developmental biology 0303 health sciences Tissue Engineering Tissue Scaffolds Mesenchymal stem cell Sugar Acids Mesenchymal Stem Cells Osteoblast General Medicine 021001 nanoscience & nanotechnology Lactic acid RUNX2 medicine.anatomical_structure chemistry Biophysics Alkaline phosphatase 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 162:523-532 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2020.06.157 |
| Popis: | Three-dimensional (3D) printing is a promising technology to fabricate the intricate biomimetic structure. The primary focus of this study was to develop the bioactive 3D-scaffolds to enhance bone regeneration. The 3D-poly (lactic acid) (PLA) scaffolds were extruded based on a computer-aided design (CAD) model and coated with gelatin (Gel) containing different concentrations of mucic acid (MA) and were investigated for their osteogenic potential. Coating the PLA scaffolds with Gel/MA improved their physicochemical properties, and the addition of MA did not alter these properties. The viability of mouse mesenchymal stem cells (mMSCs, C3H10T1/2) seeded onto the PLA/Gel/MA scaffolds remained unaffected both at metabolic and cell membrane integrity levels. Alkaline phosphatase and von Kossa staining indicated the promotion of osteoblast differentiation of mMSCs by MA in the PLA/Gel scaffolds. Inclusion of MA in PLA/Gel scaffolds also increased the expression of the master bone transcription factor, Runx2, and other osteoblastic differentiation marker genes in mMSCs. Thus, our results suggested that the 3D-printed PLA scaffolds coated with Gel/MA favor osteoblast differentiation and have potential applications in bone tissue engineering. |
| Databáze: | OpenAIRE |
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