Interleukin 6 production by human proximal tubular epithelial cells in vitro: analysis of the effects of interleukin-1 alpha (IL-1 alpha) and other cytokines
| Autor: | B. A. Yard, M. R. Daha, F. J. Van Der Woude, E. Schrama, L. A. Van Es, R. N. Boswell |
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| Rok vydání: | 1994 |
| Předmět: |
Interleukin 2
Lipopolysaccharides medicine.medical_specialty DNA Complementary Blotting Western Molecular Sequence Data Alpha (ethology) Biology Polymerase Chain Reaction Epithelium Interleukin 22 Kidney Tubules Proximal Interferon-gamma Interleukin 20 Aldesleukin Internal medicine medicine Humans Cycloheximide Cells Cultured Transplantation Base Sequence Interleukin-6 Tumor Necrosis Factor-alpha Interleukin Molecular biology Actins Recombinant Proteins Interleukin 33 Endocrinology Nephrology Interleukin-2 Tumor necrosis factor alpha medicine.drug Interleukin-1 |
| Zdroj: | Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. 9(6) |
| ISSN: | 0931-0509 |
| Popis: | Proximal tubular epithelial cells (PTEC) from human renal tissue obtained from biopsy or nephrectomy were grown in monoculture and evaluated in vitro at passage 2-4 for interleukin 6 (IL-6) production in response to medium alone or to interleukin 1 alpha (IL-1 alpha), tumour necrosis factor alpha (TNF alpha), interleukin 2 (IL-2), interferon gamma (INF gamma) or lipopolysaccharide (LPS). IL-6 bioactivity was quantitated using the IL-6-dependent murine hybridoma cell line (B9) and expressed as IL-6 units/ml/10(5) PTEC. PTEC cell lines exposed to medium alone produced intermediate amounts of IL-6 with substantial variability between cell lines. Introduction of IL-1 alpha resulted in a dose- and time-dependent increase in IL-6 production by PTEC that was maximal at 1 ng/ml IL-1 alpha at 24 h. All PTEC cell lines showed an increased IL-6 production on exposure to IL-1 alpha varying from 1.3- to 24-fold increase over baseline production. This response was completely blocked by anti-rIL-1 alpha. No significant IL-6 production by PTEC could be induced by TNF alpha, IL-2, IFN gamma, or LPS over a broad dosage range. Cycloheximide inhibited IL-6 production without irreversible cell toxicity, indicating de-novo synthesis. IL-6 produced by PTEC had a molecular weight of 26-29 kDa as demonstrated by Western blot analysis. Using PCR analysis we could demonstrate upregulation by IL-1 alpha of IL-6 mRNA in a dose-response fashion, indicating that IL-1 alpha regulates IL-6 production at a pretranslational value of protein synthesis.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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