Fermented Milk Containing Lactobacillus casei Strain Shirota Preserves the Diversity of the Gut Microbiota and Relieves Abdominal Dysfunction in Healthy Medical Students Exposed to Academic Stress
| Autor: | Kouji Miyazaki, Kazuhito Rokutan, Yusuke Gondo, Yuki Kuwano, Hiroko Kikuchi-Hayakawa, Akito Kato-Kataoka, Kensei Nishida, Kensuke Shimizu, Takahiro Matsuki, Mai Takada, Osamu Watanabe, Kazunori Suda, Hiroshi Ishikawa, Akira Kushiro, Tomoki Igarashi, Ryoutaro Hoshi, Mitsuhisa Kawai |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
DNA Bacterial Male 0301 basic medicine Lactobacillus casei Students Medical Visual analogue scale Physiology Gut flora Placebo DNA Ribosomal Applied Microbiology and Biotechnology law.invention Placebos Young Adult 03 medical and health sciences Probiotic 0302 clinical medicine Double-Blind Method Randomized controlled trial Stress Physiological law RNA Ribosomal 16S Animals Cluster Analysis Humans Young adult Bacteroidaceae Phylogeny Ecology biology Probiotics food and beverages Sequence Analysis DNA biology.organism_classification Biota Gastrointestinal Tract Lacticaseibacillus casei Milk Treatment Outcome 030104 developmental biology Fermentation Immunology Food Microbiology Female 030217 neurology & neurosurgery Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 82:3649-3658 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | Stress-induced abdominal dysfunction is an attractive target for probiotics. To investigate the effects of the probiotic Lactobacillus casei strain Shirota on abdominal dysfunction, a double-blind, placebo-controlled trial was conducted with healthy medical students undertaking an authorized nationwide examination for academic advancement. For 8 weeks, until the day before the examination, 23 and 24 subjects consumed an L. casei strain Shirota-fermented milk and a placebo milk daily, respectively. In addition to assessments of abdominal symptoms, psychophysical state, and salivary stress markers, gene expression changes in peripheral blood leukocytes and composition of the gut microbiota were analyzed using DNA microarray analysis and 16S rRNA gene amplicon sequence analysis, respectively, before and after the intervention. Stress-induced increases in a visual analog scale measuring feelings of stress, the total score of abdominal dysfunction, and the number of genes with changes in expression of more than 2-fold in leukocytes were significantly suppressed in the L. casei strain Shirota group compared with those in the placebo group. A significant increase in salivary cortisol levels before the examination was observed only in the placebo group. The administration of L. casei strain Shirota, but not placebo, significantly reduced gastrointestinal symptoms. Moreover, 16S rRNA gene amplicon sequencing demonstrated that the L. casei strain Shirota group had significantly higher numbers of species, a marker of the alpha-diversity index, in their gut microbiota and a significantly lower percentage of Bacteroidaceae than the placebo group. Our findings indicate that the daily consumption of probiotics, such as L. casei strain Shirota, preserves the diversity of the gut microbiota and may relieve stress-associated responses of abdominal dysfunction in healthy subjects exposed to stressful situations. IMPORTANCE A novel clinical trial was conducted with healthy medical students under examination stress conditions. It was demonstrated that the daily consumption of lactic acid bacteria provided health benefits to prevent the onset of stress-associated abdominal symptoms and a good change of gut microbiota in healthy medical students. |
| Databáze: | OpenAIRE |
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