Generalized indicator plate for genetic, metabolic, and taxonomic studies with microorganisms
| Autor: | B R Bochner, Michael A. Savageau |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Salmonella typhimurium
food.ingredient Microorganism Tetrazolium Salts Saccharomyces cerevisiae medicine.disease_cause Applied Microbiology and Biotechnology chemistry.chemical_compound food Pseudomonas medicine Escherichia coli Agar Histidine Color formation Ecology biology Acinetobacter Bacteria Tetrazolium chloride Fungi biology.organism_classification Phenotype chemistry Biochemistry Mutation Indicators and Reagents Formazan Food Science Biotechnology Research Article |
| Zdroj: | Applied and environmental microbiology. 33(2) |
| ISSN: | 0099-2240 |
| Popis: | We have developed an indicator plate that works well for diverse types of substrates and microorganisms. The plates are inexpensive and easy to prepare. The essential components are agar, buffer, growth-supporting nutrients, a test substrate, and 2,3,5-triphenyl tetrazolium chloride (TTC). Using various strains of Salmonella typhimurium and Escherichia coli, we have studied and defined the contribution of each component to the satisfactory function of the plate. Colonies capable of catabolizing the test substrate reduce TTC and produce a deep red formazan, whereas colonies failing to catabolize the substrate remain uncoloured. Those with intermediate rates of catabolism differ in rate and/or extent of color formation. In all cases the color is stable because TTC reduction is essentially irreversible. Since the mode of action of these plates is fairly well understood, alternative formulations can be devised to meet specific needs. The general applicability of this TTC indicator system makes it an extremely useful tool in microbial genetics, metabolism, and taxonomy. |
| Databáze: | OpenAIRE |
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