Characterization and evaluation of a novel immunochromatographic assay for pharyngeal Mycoplasma pneumoniae ribosomal protein L7/L12 antigens
| Autor: | Yoshikazu Ishii, Naruhiko Ishiwada, Katsuya Watanabe, Sakae Homma, Keita Matsubara, Tsutomu Itagaki, Kazuhiro Tateda, Yoshitaka Nakamori, Kenji Matsuyama, Satoshi Iwata, Go Sano |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Ribosomal Proteins 0301 basic medicine Microbiology (medical) Mycoplasma pneumoniae Microbiological culture Point-of-Care Systems 030106 microbiology Biology medicine.disease_cause Sensitivity and Specificity Microbiology Chromatography Affinity Mice Young Adult 03 medical and health sciences Mycoplasma Japan Antigen Pneumonia Mycoplasma medicine Animals Humans Child Aged Antigens Bacterial Pharynx General Medicine Middle Aged medicine.disease In vitro Pneumonia Upper respiratory tract infection medicine.anatomical_structure Child Preschool embryonic structures Female |
| Zdroj: | Journal of Medical Microbiology. 65:1105-1110 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Point-of-care testing for Mycoplasma pneumoniae infection may be ideal and useful because significant numbers of the cases will be seen as outpatients. Recently, a new immunochromatographic method (ICM) targeting M. pneumoniae ribosomal protein L7/L12 (RP-L7/L12) in pharyngeal swabs became available in Japan, although clinical data and basic information regarding efficacy and characterization of this ICM are limited. The present study examined the fate of M. pneumoniae RP-L7/L12 during in vitro growth and the correlation between M. pneumoniae concentration in clinical specimens and the sensitivity of the ICM test. The usefulness of the ICM was investigated in patients suspected of having M. pneumoniae pneumonia and upper respiratory tract infection (137 children and 39 adults). The limit of detection for the ICM test was 1.1×104 c.f.u. ml-1 of M. pneumoniae. Bacterial production of RP-L7/L12 correlated positively with the viable M. pneumoniae concentration in vitro; antigen was then degraded in culture broth, with an in vitro half-life of approximately 2 days. Five other Mycoplasma spp. and 14 representative respiratory pathogens were ICM assay negative at bacterial concentrations of 106 c.f.u. ml-1. The clinical sensitivity and specificity of the ICM assay were 57.1 % (20/35) and 92.2 % (130/141), respectively, in comparison with bacterial culture. Clinical specimens containing ≥106 c.f.u. ml-1 of M. pneumoniae burden were ICM positive in 13 of 18 cases (72.2 %). The ICM is a poorly sensitive but reasonably specific means for detecting M. pneumoniae infections. |
| Databáze: | OpenAIRE |
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