Effects of 4,4'-Diisothiocyanato-stilbene-2,2'-disulfonic Acid (DIDS) and Chlorpromazine on NO3− Transport via Anion Exchanger in Erythrocytes: Inertness of DIDS in Whole Blood
| Autor: | Matomo Nishio, Kaname Kubota, Taku Matsubara, Masaru Yamazoe, Kazuyuki Ozaki, Takaharu Ishibashi, Tomoyuki Hori, Junko Yoshida, Yoshifusa Aizawa |
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| Rok vydání: | 2003 |
| Předmět: |
Adult
Anions Male inorganic chemicals Erythrocytes Time Factors Chlorpromazine 4 4'-Diisothiocyanostilbene-2 2'-Disulfonic Acid In Vitro Techniques chemistry.chemical_compound Anion Exchange Protein 1 Erythrocyte Extracellular fluid medicine Humans Anion exchanger Whole blood Pharmacology Nitrates Chromatography Chemistry lcsh:RM1-950 Albumin food and beverages Biological Transport Extracellular Fluid Middle Aged lcsh:Therapeutics. Pharmacology DIDS Molecular Medicine Female Antipsychotic Agents medicine.drug |
| Zdroj: | Journal of Pharmacological Sciences, Vol 93, Iss 4, Pp 505-508 (2003) |
| ISSN: | 1347-8648 1347-8613 |
| DOI: | 10.1254/jphs.93.505 |
| Popis: | We examined the effects of chlorpromazine on NO(3)(-) transport between erythrocytes (RBCs) and extracellular fluid. Chlorpromazine (10 microg/ml) did not influence NO(3)(-) movement in both whole blood and RBC suspension. Though an anion exchanger (AE1) inhibitor DIDS (4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid, 100 microM) did not alter NO(3)(-) movement in whole blood, it inhibited the movement in a concentration-dependent manner in the RBC suspension. The inhibition was abrogated by plasma and albumin concentration-dependently. Our results indicated that chlorpromazine had no effect on NO(3)(-) transport through AE1 and that the inertness of DIDS on AE1 in whole blood is due to interference by albumin in plasma. |
| Databáze: | OpenAIRE |
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