Participation of rhop21 in serum-dependent invasion by rat ascites hepatoma cells
| Autor: | Mukai Mutsuko, Ogawa Haruki, Kiyoko Yoshioka, Akedo Hitoshi, Komagome Rika, Imamura Fumio, Shinkai Kiyoko, Miyoshi Jun |
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| Rok vydání: | 1995 |
| Předmět: |
rho GTP-Binding Proteins
Rat ascites hepatoma cell RHOA Botulinum Toxins Molecular Sequence Data Biophysics Biology Transfection Rhop21 Biochemistry Epithelium chemistry.chemical_compound Liver Neoplasms Experimental Invasion Structural Biology Cell Movement GTP-Binding Proteins Complementary DNA Ascites Lysophosphatidic acid Genetics medicine Tumor Cells Cultured Animals Humans Point Mutation Neoplasm Invasiveness RNA Messenger Activated rhoA Molecular Biology ADP Ribose Transferases Fetus Base Sequence Rats Inbred Strains Cell Biology Transfectant Molecular biology Culture Media Rats Blood chemistry biology.protein Experimental pathology Cattle medicine.symptom Mesothelial Cell |
| Zdroj: | FEBS letters. 372(1) |
| ISSN: | 0014-5793 |
| Popis: | Rat ascites hepatoma cells (MM1 cells) penetrate through a cultured mesothelial cell monolayer (MCL) in the presence of fetal calf serum (FCS), but scarcely do so in its absence. Inactivation of rhop21 of MM1 cells by ADP-ribosyltransferase C3 resulted in the suppression of this serum effect on the penetration, suggesting that the serum effect was mediated by rhop21. To ascertain this assumption MM1 cells were transfected with an activated (Val14) human rhoA cDNA (Neo/RhoA 1–7). The transfectants penetrated MCL extensively even in the absence of FCS and became largely independent of serum for the penetration. These results suggest that serum-induced invasion by MM1 cells is mainly mediated by rhop21. |
| Databáze: | OpenAIRE |
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