Increase in proteins involved in mitochondrial fission, mitophagy, proteolysis and antioxidant response in type I endometrial cancer as an adaptive response to respiratory complex I deficiency
Autor: | Maria Nicola Gadaleta, Clara Musicco, Giuseppe Gasparre, Gennaro Cormio, Antonella Cormio, Vito Pesce, Anna Maria Sardanelli |
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Přispěvatelé: | Cormio, Antonella, Musicco, Clara, Gasparre, Giuseppe, Cormio, Gennaro, Pesce, Vito, Sardanelli, Annamaria, Gadaleta, Maria Nicola |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
BNIP3 CLPP Biophysics Mitochondrial Degradation MFN2 Endometrial carcinoma Mitochondrion Biology Biochemistry Antioxidants Mitochondrial Proteins 03 medical and health sciences ALR Complex I deficiency Mitochondrial dynamics Mitophagy Tumor Cells Cultured Humans Molecular Biology Electron Transport Complex I Cell Biology Fusion protein Endometrial Neoplasms Mitochondria Neoplasm Proteins Cell biology 030104 developmental biology Proteolysis Cancer cell DNAJA3 Female Mitochondrial fission Reactive Oxygen Species |
Zdroj: | Biochemical and biophysical research communications 491 (2017): 85–90. doi:10.1016/j.bbrc.2017.07.047 info:cnr-pdr/source/autori:1Cormio, Antonella; 2Musicco, Clara; 3Gasparre, Giuseppe; 4Cormio, Gennaro; 1Pesce, Vito; 5Sardanelli, Anna Maria; 1Gadaleta, Maria Nicola/titolo:Increase in proteins involved in mitochondrial fission, mitophagy, proteolysis and antioxidant response in type I endometrial cancer as an adaptive response to respiratory complex I deficiency./doi:10.1016%2Fj.bbrc.2017.07.047/rivista:Biochemical and biophysical research communications (Print)/anno:2017/pagina_da:85/pagina_a:90/intervallo_pagine:85–90/volume:491 |
ISSN: | 0006-291X |
DOI: | 10.1016/j.bbrc.2017.07.047 |
Popis: | Pathogenic mtDNA mutations associated with alterations of respiratory complex I, mitochondrial proliferation (oncocytic-like phenotype) and increase in antioxidant response were previously reported in type I endometrial carcinoma (EC). To evaluate whether in the presence of pathogenic mtDNA mutations other mitochondrial adaptive processes were triggered by cancer cells, the expression level of proteins involved in mitochondrial dynamics, mitophagy, proteolysis and apoptosis were evaluated in type I ECs harboring pathogenic mtDNA mutations and complex I deficiency. An increase in the fission protein Drp1, in the mitophagy protein BNIP3, in the mitochondrial protease CLPP, in the antioxidant and anti-apoptotic protein ALR and in Bcl-2 as well as a decrease in the fusion protein Mfn2 were found in cancer compared to matched non malignant tissue. Moreover, the level of these proteins was measured in type I EC, in hyperplastic (the premalignant form) and in non malignant tissues to verify whether the altered expression of these proteins is a common feature of endometrial cancer and eventually of hyperplastic tissue. This analysis confirmed in type I EC samples, but not in hyperplasia, an alteration of the expression level of these proteins. These results suggest that in this cancer mitochondrial fission, antioxidant and anti-apoptotic response may be activated, as well as the discharge of damaged mitochondrial proteins as mitochondrial adaptation processes to mitochondrial dysfunction. |
Databáze: | OpenAIRE |
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