Contemporary Approaches for Site‐selective Dual Functionalization of Proteins
| Autor: | Lujuan Xu, Seah Ling Kuan, Tanja Weil |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
biomedical applications
Models Molecular Reviews Nanotechnology Review 010402 general chemistry 01 natural sciences Catalysis site-selective protein modification protein conjugates Protein structure Fluorescence Resonance Energy Transfer Site selective Amino Acids Protein therapeutics Future perspective Molecular Structure 010405 organic chemistry Chemistry Proteins General Medicine General Chemistry DUAL (cognitive architecture) 0104 chemical sciences Folding (chemistry) Crosstalk (biology) protein dual functionalization Surface modification Protein Functionalization |
| Zdroj: | Angewandte Chemie International Edition Angewandte Chemie (International Ed. in English) |
| Popis: | Site‐selective protein functionalization serves as an invaluable tool for investigating protein structures and functions in complicated cellular environments and accomplishing semi‐synthetic protein conjugates such as traceable therapeutics with improved features. Dual functionalization of proteins allows the incorporation of two different types of functionalities at distinct location(s), which greatly expands the features of native proteins. The attachment and crosstalk of a fluorescence donor and an acceptor dye provides fundamental insights into the folding and structural changes of proteins upon ligand binding in their native cellular environments. Moreover, the combination of drug molecules with different modes of action, imaging agents or stabilizing polymers provides new avenues to design precision protein therapeutics in a reproducible and well‐characterizable fashion. This review aims to give a timely overview of the recent advancements and a future perspective of this relatively new research area. First, the chemical toolbox for dual functionalization of proteins is discussed and compared. The strengths and limitations of each strategy are summarized in order to enable readers to select the most appropriate method for their envisaged applications. Thereafter, representative applications of these dual‐modified protein bioconjugates benefiting from the synergistic/additive properties of the two synthetic moieties are highlighted. Site‐selective dual protein functionalization serves as an invaluable tool for investigating protein structures and functions in complicated cellular environments and accomplishing semi‐synthetic protein conjugates. The methodologies, as well as the applications of dual‐functionalized protein bioconjugates are highlighted in this review. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|