No effect of remote ischaemic conditioning on inflammation in a porcine kidney transplantation model
| Autor: | Ulla Moeldrup, Troels M. Joergensen, Asger Granfeldt, Christoffer Soelling, Bente Jespersen, Anna Krarup Keller, Niels Jørgen Secher, Ernst O. Ostraat, P. Soendergaard, Else Toennesen, Kristian Ravlo, Lise Wogensen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Neutrophils Swine medicine.medical_treatment Interleukin-1beta Immunology Delayed Graft Function Apoptosis Inflammation Kidney Gastroenterology Pathogenesis Random Allocation Internal medicine medicine Animals Immunology and Allergy Kidney transplantation Transplantation Interleukin-6 Tumor Necrosis Factor-alpha business.industry Macrophages Cold Ischemia Graft Survival Interleukin-8 medicine.disease Kidney Transplantation Interleukin-10 Cytokine medicine.anatomical_structure surgical procedures operative Neutrophil Infiltration Models Animal medicine.symptom business Infiltration (medical) |
| Zdroj: | Secher, N, Soendergaard, P, Ravlo, K, Soelling, C, Granfeldt, A, Wogensen, L, Keller, A K, Moeldrup, U, Ostraat, E O, Joergensen, T M, Jespersen, B & Toennesen, E 2014, ' No effect of remote ischaemic conditioning on inflammation in a porcine kidney transplantation model ', Transplant Immunology, vol. 31, no. 2, pp. 98-104 . https://doi.org/10.1016/j.trim.2014.05.005 |
| Popis: | Main problem Delayed graft function after kidney transplantation is associated with decreased graft survival and increased patient mortality but the pathogenesis is poorly understood. Remote ischaemic conditioning (rIC) may prevent delayed graft function by an anti-inflammatory effect. In a porcine model of transplantation from adults to children, we investigated the inflammatory response in the transplanted kidney and the effect of rIC. Methods Kidneys were recovered from brain dead donor pigs(63 kg) and transplanted into two groups of recipient pigs(15 kg) after 22 h of cold ischaemia. Recipients were randomised to either: rIC (n = 8) performed before the 10-h reperfusion period or no-rIC (n = 8). Non-transplanted kidneys from eight brain dead pigs served as controls. Results Compared to controls, transplantation increased the number of apoptotic cells, macrophages and neutrophils in the kidney. After transplantation, IL-10 levels increased and IL-6 levels decreased in the kidney, whereas levels of TNF-α and IL-8 were not affected. A significant rise in plasma IL-1β and IL-6 was observed in the recipients after transplantation. Plasma IL-10 was not affected by transplantation and TNF-α and IL-8 were below detection limit. No effect of rIC was found with regards to cell infiltration or cytokine production. Conclusion Renal transplantation elicits an inflammatory response in the kidney manifested as apoptotic cell death, macrophage and neutrophil infiltration, and an anti-inflammatory cytokine response 10 h after transplantation. This response was not modified by rIC. |
| Databáze: | OpenAIRE |
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