In vitro maturation in the presence of Leukemia Inhibitory Factor modulates gene and miRNA expression in bovine oocytes and embryos
| Autor: | Meritxell Vendrell-Flotats, Teresa Mogas, Iris Martínez-Rodero, Tania García-Martínez, Manel Lopez-Bejar, Marc Yeste, Jonathan LaMarre |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell lcsh:Medicine Leukemia Inhibitory Factor 0302 clinical medicine Gene expression Animals -- Cria i desenvolupament lcsh:Science Cells Cultured reproductive and urinary physiology 2. Zero hunger Cumulus Cells 030219 obstetrics & reproductive medicine Multidisciplinary Chemistry Reproduction Gene Expression Regulation Developmental Cell Differentiation Embryo medicine.anatomical_structure embryonic structures Female hormones hormone substitutes and hormone antagonists endocrine system Fertilization in Vitro Article Andrology 03 medical and health sciences Regulació genètica medicine Animals Blastocyst Animal breeding Genetic regulation urogenital system lcsh:R Factor inhibidor de la leucèmia Embryo Mammalian Oocyte Expressió gènica In Vitro Oocyte Maturation Techniques HSPA1A In vitro maturation Gene regulation MicroRNAs 030104 developmental biology Leukemia inhibitory factor Oocytes Cattle lcsh:Q |
| Zdroj: | Scientific Reports, 2020, vol. 10, núm. art.17777 Articles publicats (D-B) DUGiDocs – Universitat de Girona instname Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Scientific Reports |
| Popis: | Members of the interleukin-6 (IL-6) family of cytokines are important for reproductive function that are mediated through changes in gene and miRNA expression. Herein, we characterized the expression of miR-21, miR-155, miR-34c and miR-146a in bovine oocytes and cumulus cells during in vitro maturation (IVM) with leukemia inhibitory factor (LIF), IL-6 and IL-11 or unsupplemented controls. LIF-exposed COCs showed higher expression of miR-21 and miR-155 in oocytes, whereas miR-146a expression was increased in oocytes matured with IL-6 and IL-11. In cumulus cells, miR-155 expression was elevated by all treatments while only LIF increased miR-21 expression. Based on these results, we next examined how LIF exposure during IVM affected oocyte competence, through IVF and the expression of specific genes in GV- and MII-oocytes, in 2- and 8-cell embryos, and in Day 8-blastocysts. LIF supplementation did not affect cleavage rate, blastocyst yield or several other developmental parameters, but did increase hatching rate. LIF suppressed DPPA3, ZAR1 and NPM2 expression in 2 cell- and/or 8-cell embryos. LIF increased the expression of KAT2A and HSPA1A in MII-oocytes, and that of HDAC1, KAT2A and HSP90AA1 and the BAX:BCL2L1 ratio in 2-cell embryos. In contrast, HDAC1, KAT2A and HSP90AA1 expression and BAX:BCL2L1 ratio was lower in 8-cell embryos derived from LIF oocytes. IVM with LIF also increased the expression of DNMT3A, HSPA1A and HSP90AA1 in blastocysts. In conclusion, supplementation with LIF during IVM was consistently associated with changes in the relative abundance of transcripts in mature bovine oocytes and in specific embryo developmental stages Tis study was supported by the Spanish Ministry of Science and Innovation (Project AGL2016-79802-P and grant CTQ2014-59632-R) and the Generalitat de Catalunya (Project No. 2017 SGR 1229). Dr. Mertixell VendrellFlotats was supported by an FPI scholarship (BES-2014-071075). Collaboration with Dr. LaMarre was supported by a Fellowship under the OECD Co-operative Research Programme: Biological Resource Management for Sustainable Agricultural Systems and funded by NSERC (Canada) |
| Databáze: | OpenAIRE |
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