Comparison of the Antithrombotic Effect of PEG-Hirudin and Heparin in a Human Ex Vivo Model of Arterial Thrombosis
| Autor: | Kjell S. Sakariassen, Jean-Pierre Bossavy, Bernard Boneu, Yves Cadroy, K. Rübsamen, Claire Thalamas |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.drug_class
Antithrombin III Hirudin Arterial Occlusive Diseases Sodium Chloride Pharmacology Thromboplastin Fibrin Fibrinogen Degradation Products Fibrinolytic Agents PEG ratio medicine Humans Platelet Blood Coagulation Fibrin medicine.diagnostic_test Heparin business.industry Anticoagulant Thrombosis Hirudins Platelet Activation beta-Thromboglobulin P-Selectin Anesthesia Blood Coagulation Tests Glass Cardiology and Cardiovascular Medicine business Perfusion Ex vivo Half-Life Peptide Hydrolases medicine.drug Partial thromboplastin time |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 19:1348-1353 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/01.atv.19.5.1348 |
| Popis: | Abstract —Polyethylene glycol (PEG)-hirudin is a derivative of hirudin with a long plasma half-life. We have compared the efficacy of PEG-hirudin with unfractionated heparin (UH) in preventing arterial thrombosis. Arterial thrombus formation was induced ex vivo in 12 healthy human volunteers by exposing a tissue factor–coated coverslip positioned in a parallel-plate perfusion chamber to flowing nonanticoagulated human blood drawn directly from an antecubital vein at an arterial wall shear rate of 2600 s −1 for 3.5 minutes. PEG-hirudin, UH, or saline (as control) were administered ex vivo through a heparin-coated mixing device positioned proximal to the perfusion chamber. Platelet and fibrin deposition was quantified by immunoenzymatic measure of the P-selectin and d -dimer content of dissolved plasmin-digested thrombi, respectively. UH was administered to a plasma concentration of 0.35 IU/mL. This concentration prolonged the activated partial thromboplastin time from 32±1 seconds to 79±4 seconds ( P P 80% reduction at 5 μg/mL ( P |
| Databáze: | OpenAIRE |
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