Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
| Autor: | Che Abdullah Abu-Bakar, Mohd Ikhsan Khalid, Syazana Aimi, Son Radu, John Yew Huat Tang |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Veterinary medicine lcsh:Arctic medicine. Tropical medicine Antibiotic resistance lcsh:RC955-962 030106 microbiology Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Campylobacter jejuni 03 medical and health sciences Ampicillin medicine Enrofloxacin RAPD analysis Agar diffusion test lcsh:QH301-705.5 business.industry Multiple antibiotic resistance index biology.organism_classification bacterial infections and mycoses Biotechnology RAPD Ciprofloxacin lcsh:Biology (General) Amikacin business medicine.drug |
| Zdroj: | Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 1, Pp 71-75 (2016) |
| ISSN: | 2221-1691 |
| Popis: | Objective To investigate antibiotic resistance profile and characterize Campylobacter jejuni ( C. jejuni ) isolates using random amplified polymorphic DNA (RAPD) analysis. Methods Ninety eight C. jejuni isolates from farms and retail outlets were screened against 10 antibiotics commonly used clinically and agriculturally by using disk diffusion method. RAPD analysis was done to characterize 98 C. jejuni isolates. Results Fifty-one percent of the isolates had multiple antibiotic resistance index 0.2 and below. This indicated that the isolates in the vegetables were not from the high risk environment or extensive farming practices. C. jejuni isolates found resistant towards penicillin G (93%), vancomycin (86%), ampicillin (35%), erythromycin (28%), gentamycin (4%), amikacin (3%), enrofloxacin (1%), norfloxacin (1%) and no resistance towards ciprofloxacin. RAPD clustering analysis showed that the contamination of C. jejuni in vegetables was likely due to cross contamination at retail markets. Conclusions C. jejuni contamination in vegetables at retail markets was due to cross contamination. Current finding proved that C. jejuni in small scale vegetables production was less expose towards antibiotic abuse. |
| Databáze: | OpenAIRE |
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