An in planta induced gene of Phytophthora infestans codes for ubiquitin
| Autor: | Corné M. J. Pieterse, Eddy Risseeuw, L. C. Davidse |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Phytophthora
Sequence analysis Phytophthora infestans Molecular Sequence Data Plant Science macromolecular substances Fungal Proteins polyubiquitin gene Complementary DNA Gene expression ubiquitin Genetics Genomic library Amino Acid Sequence Cloning Molecular Ubiquitins Gene Solanum tuberosum Southern blot Base Sequence biology fungi RNA food and beverages General Medicine Blotting Northern biology.organism_classification Molecular biology Laboratorium voor Phytopathologie Blotting Southern Gene Expression Regulation Oomycetes Multigene Family Laboratory of Phytopathology differential screening DNA Probes potato late blight Agronomy and Crop Science Biologie |
| Zdroj: | Current plant science and biotechnology in agriculture, 10, 272. Kluwer Academic Publishers Plant Molecular Biology 17 (1991) Plant molecular biology, 17, 799. Kluwer Academic Publishers Plant Molecular Biology, 17, 799-811 |
| ISSN: | 0167-4412 |
| Popis: | An in planta induced gene of Phytophthora infestans (the causal organism of potato late blight) was selected from a genomic library by differential hybridization using labelled cDNA derived from poly(A)+ RNA of P. infestans grown in vitro and labelled cDNA made from potato-P. infestans interaction poly(A)+ RNA as probes. Sequence analysis showed that the gene codes for ubiquitin, a highly conserved protein which plays an important role in several cellular processes. The structure of the polyubiquitin gene (designated ubi3R) is consistent with the structure of other known polyubiquitin genes. It consists of three repeats in a head-to-tail arrangement without intervening sequences, each encoding a ubiquitin unit of 76 amino acids. The last ubiquitin unit is followed by an extra asparagine residue at the carboxy-terminal end. Northern and Southern blot analyses revealed that the polyubiquitin gene is a member of a multigene family, all genes of which show induced expression in planta. |
| Databáze: | OpenAIRE |
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