Antioxidant enzyme response of sorghum plant upon exposure to Aluminum, Chromium and Lead heavy metals
| Autor: | Mahmut Kaplan, Semih Yilmaz, Seyma Hava Yilmaz, Ridvan Temizgul |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Antioxidant medicine.medical_treatment Clinical Biochemistry chemistry.chemical_element 010501 environmental sciences 01 natural sciences Biochemistry chemistry.chemical_compound Chromium Aluminium medicine Proline Molecular Biology 0105 earth and related environmental sciences chemistry.chemical_classification biology Biochemistry (medical) food and beverages Heavy metals Sorghum biology.organism_classification Enzyme chemistry Agronomy Environmental chemistry Chlorophyll 010606 plant biology & botany |
| Zdroj: | Turkish Journal of Biochemistry. 42 |
| ISSN: | 1303-829X |
| Popis: | Objective:Sorghum has received great interest for resistance to heavy metals. Therefore, effects of Aluminum (Al), Chromium (Cr) and Lead (Pb) concentrations (2, 4, 8, 16, 32 and 64 ppm) on antioxidant enzyme systems of Sorghum in root and leaf tissues were investigated.Methods:Seeds were cultivated in hydroponic Hoagland solution containing heavy metal concentrations in a growth chamber. Malondialdehyde (MDA), proline levels, catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR) and glutathione S-transferase (GST) activities were evaluated after treatment.Results:Al doses decreased chlorophyll a (Chl a) at 4 ppm and subsequent doses, and total Chl at 32 and 64 ppm doses, however, it did not cause any change at Chl b except at 2 ppm. Although 64 ppm of Al, Cr, and Pb decreased total Chl, it increased proline level (nearly 5, 21 and 40 times higher compared to control, respectively) in leaf. Common observation is that positive correlation was apparent between proline, MDA, APX and GST activity for Al and Cr treatments and GST activity for three elements in root. Also, the only positive correlation was evident between proline and MDA for Al and Cr applications in leaf.Conclusion:Although sorghum is resistant to heavy metals, induction of antioxidant enzymes seem to be not enough at higher concentrations to protect cells from heavy metal toxicity; however, it has great importance for further studies to find out whether phenolic compounds produced in sorghum have additive role in this regard. |
| Databáze: | OpenAIRE |
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