Leishmania infantum DNA detection in Phlebotomus tobbi in a new northern focus of visceral leishmaniasis in Iran
| Autor: | S Rafizadeh, Yavar Rassi, Yagoobi-Ershadi, Abbas Bahrami, Amir Ahmad Akhavan, Nasrollah Maleki, Mohammad Ali Oshaghi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Veterinary medicine Vector(s) lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 lcsh:Medicine Alborz Iran Phlebotomus tobbi law.invention law parasitic diseases medicine Parasite hosting Leishmania infantum Polymerase chain reaction biology lcsh:R medicine.disease biology.organism_classification Virology Dna detection Infectious Diseases Visceral leishmaniasis Vector (epidemiology) Restriction fragment length polymorphism Basic Researches |
| Zdroj: | Asian Pacific Journal of Tropical Disease, Vol 4, Iss 2, Pp 110-114 (2014) |
| ISSN: | 2222-1808 |
| Popis: | Objective To identify the vector(s), the parasite and the species composition of sand flies in the district during May–October 2012. Methods For reaching our objectives we used polymerase chain reaction of kDNA, ITS1-rDNA, followed by restriction fragment length polymorphism. Results Two species of Phlebotomus sergenti and Phlebotomus tobbi were the most prevalent among 8 species identified comprising 51.1% and 32.9% respectively. Among the 160 specimens of female sand flies tested by polymerase chain reaction of kDNA, ITS1-rDNA, followed by restriction fragment length polymorphisms, only 1 out of 80 Phlebotomus tobbi (1.25%) were positive to Leishmania infantum parasites. Conclusions Our finding showed that Phlebotomus tobbi may play as a vector to circulate the parasite of Leishmania infantum among reservoir(s) and human. |
| Databáze: | OpenAIRE |
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