Hair cell counts in a rat model of sound damage: Effects of tissue preparationidentification of regions of hair cell loss
| Autor: | Hinrich Staecker, Dianne Durham, Stefanie Kennon-McGill, Andrea Freemyer, Axel Shum, Christopher A Neal |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology medicine.medical_specialty Hearing loss Article Sound exposure Hair Cells Auditory medicine otorhinolaryngologic diseases Animals Rats Long-Evans Cochlea Sound (medical instrument) Hair Cells Auditory Inner business.industry Hyperacusis Reproducibility of Results Auditory Threshold Anatomy medicine.disease Sensory Systems Rats Hair Cells Auditory Outer medicine.anatomical_structure Sound Hearing Loss Noise-Induced Hair cell sense organs Tissue Preservation medicine.symptom business Noise Plastics Tinnitus Noise-induced hearing loss |
| Zdroj: | Hearing research. 328 |
| ISSN: | 1878-5891 |
| Popis: | Exposure to intense sound can damage or kill cochlear hair cells (HC). This loss of input typically manifests as noise induced hearing loss, but it can also be involved in the initiation of other auditory disorders such as tinnitus or hyperacusis. In this study we quantify changes in HC number following exposure to one of four sound damage paradigms. We exposed adult, anesthetized Long-Evans rats to a unilateral 16 kHz pure tone that varied in intensity (114 dB or 118 dB) and duration (1, 2, or 4 h) and sacrificed animals 2-4 weeks later. We compared two different methods of tissue preparation, plastic embedding/sectioning and whole mount dissection, for quantifying hair cell loss as a function of frequency. We found that the two methods of tissue preparation produced largely comparable cochleograms, with whole mount dissections allowing a more rapid evaluation of hair cell number. Both inner and outer hair cell loss was observed throughout the length of the cochlea irrespective of sound damage paradigm. Inner HC loss was either equal to or greater than outer HC loss. Increasing the duration of sound exposures resulted in more severe HC loss, which included all HC lesions observed in an analogous shorter duration exposure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect