Exploring Epithelial–Mesenchymal Transition signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes
| Autor: | Giorgia Scarfò, Vito Cela, Claudia Martini, Francesca Vergine, Francesca Papini, Deborah Pietrobono, Ilaria Marzi, Simona Daniele, Elena Pisacreta, Maria Elena Rosa Obino, Elisa Malacarne, Elisa Zappelli, Paolo Giovanni Artini, Ferdinando Franzoni |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
endometriosis
In vitro fertilisation business.industry QH301-705.5 inflammatory cytokines Peritoneal fluid medicine.medical_treatment Endometriosis EMT Medicine (miscellaneous) Embryo medicine.disease Follicular fluid Article General Biochemistry Genetics and Molecular Biology follicular fluid Proinflammatory cytokine Epithelial–Mesenchymal Transition Pathogenesis Andrology embryonic structures medicine Epithelial–mesenchymal transition Biology (General) business |
| Zdroj: | Biomedicines Volume 9 Issue 11 Biomedicines, Vol 9, Iss 1681, p 1681 (2021) |
| ISSN: | 2227-9059 |
| DOI: | 10.3390/biomedicines9111681 |
| Popis: | Endometriosis (EMS) pathogenesis has been related to the release of inflammatory mediators in peritoneal fluid, creating an altered microenvironment that leads to low-grade oocyte/embryos and to the reduction of implantation rates. The Epithelial–Mesenchymal Transition (EMT), an inflammation-related process, can be a further contributing factor to EMS. This study aimed to investigate, among various cytokines and EMT markers (Cadherins, TGF-β, HIF-1α), diagnostic markers of EMS and prognostic factors of in vitro fertilization (IVF) outcomes. Herein, EMS patients manifested higher serum levels of the inflammatory molecules IL-6, IL-8, and IL-12 and a decrease in the concentrations of the anti-inflammatory IL-10. Moreover, biochemical markers associated with the EMT process were more elevated in serum and follicular fluid (FF) of EMS patients than in controls. At the end, the number of good-quality embryos was inversely related to serum IL-6 and EMT markers. Interestingly, serum IL-6 and FF IL-10 concentrations differentiated EMS patients from controls. Finally, serum IL-8 and E-Cadherin levels, as well as FF IL-10, predicted positive IVF outcome with great accuracy. Our data confirm the pivotal role of inflammatory mediators (i.e., IL-6 and IL-10) in EMS pathogenesis and suggest that EMT-related markers are elevated in EMS patients and can be predictive of IVF outcome. |
| Databáze: | OpenAIRE |
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