The envelope gene and long terminal repeat sequences contribute to the pathogenic phenotype of helper-independent Friend viruses
| Autor: | K Signorelli, A Oliff, L Collins |
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| Jazyk: | angličtina |
| Rok vydání: | 1984 |
| Předmět: |
Genes
Viral viruses Immunology Mice Inbred Strains Microbiology Virus Mice Retrovirus Viral envelope Viral Envelope Proteins Virology hemic and lymphatic diseases Murine leukemia virus medicine Animals Cloning Molecular Gene Cells Cultured Repetitive Sequences Nucleic Acid Leukemia Experimental biology Base Sequence DNA Restriction Enzymes Provirus biology.organism_classification medicine.disease Molecular biology Long terminal repeat Friend murine leukemia virus Leukemia Phenotype Genes Insect Science DNA Viral Research Article |
| Popis: | Friend murine leukemia virus (F-MuLV) and Friend mink cell focus-inducing virus (Fr-MCF) are helper-independent murine retroviruses which induce a rapidly fatal erytholeukemia in NIH Swiss mice. Amphotropic clone 4070 (Ampho) is a murine retrovirus which does not cause leukemia in these animals. Mice inoculated with Ampho, an Fr-MCF/Ampho pseudotype, or F-MuLV developed leukemia in 0, 50, and 100% of animals, respectively. To identify the F-MuLV and Fr-MCF sequences responsible for leukemia, we constructed hybrid viral genomes between these viruses and Ampho, using subgenomic fragments of molecularly cloned viral DNA. Transfection of these hybrid viral DNAs into fibroblasts produces recombinant retroviruses. These new viruses are assayed in vivo for their ability to cause leukemia. Recombinant viruses constructed between the Ampho genome and the Fr-MCF envelope gene do not cause leukemia. Similarly, viruses constructed by using either the Fr-MCF long terminal repeat U3 region or the F-MuLV long terminal repeat U3 region and the remainder of the Ampho genome do not cause leukemia. However, if the Fr-MCF envelope gene plus the Fr-MCF U3 region are joined to Ampho, the resulting virus causes erythroleukemia in 14% of mice. Recombinant viruses made between the Fr-MCF envelope gene, the F-MuLV U3 region, and the remainder of the Ampho genome cause erythroleukemia in 38% of mice. This study demonstrates that both the envelope gene of Fr-MCF and the U3 regions of Fr-MCF and F-MuLV contain sequences which contribute to the leukemic phenotype of helper-independent Friend viruses. |
| Databáze: | OpenAIRE |
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