Wisconsin microbiome study, a cross-sectional investigation of dietary fibre, microbiome composition and antibiotic-resistant organisms: rationale and methods
| Autor: | Megan Duster, Ronald E. Gangnon, Garret Suen, Keith P. Poulsen, Ashley E. Kates, Paul E. Peppard, Ajay K. Sethi, Daniel Shirley, Kristen Malecki, Nasia Safdar, Sanjay K. Shukla, Shoshannah Eggers, Julie A. Mares |
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| Rok vydání: | 2018 |
| Předmět: |
Dietary Fiber
Male 0301 basic medicine Gut flora medicine.disease_cause Drug Resistance Multiple Bacterial Protocol molecular biology Skin Aged 80 and over education.field_of_study biology Microbiota public health Gastrointestinal Microbiome Bacterial Infections General Medicine Middle Aged Anti-Bacterial Agents Infectious Diseases Female epidemiology Adult Methicillin-Resistant Staphylococcus aureus Adolescent 030106 microbiology Population Young Adult 03 medical and health sciences Wisconsin Antibiotic resistance Environmental health medicine Humans Microbiome education Aged Mucous Membrane business.industry microbiology Feeding Behavior biology.organism_classification Methicillin-resistant Staphylococcus aureus Diet Colonisation Cross-Sectional Studies Biorepository business |
| Zdroj: | BMJ Open |
| ISSN: | 2044-6055 |
| Popis: | IntroductionPrevention of multidrug-resistant organism (MDRO) infections, such as those caused by methicillin-resistantStaphylococcus aureus, vancomycin-resistant enterococci, fluoroquinolone-resistant Gram-negative bacteria andClostridium difficileis crucial. Evidence suggests that dietary fibre increases gut microbial diversity, which may help prevent colonisation and subsequent infection by MDROs. The aim of the Winning the War on Antibiotic Resistance (WARRIOR) project is to examine associations of dietary fibre consumption with the composition of the gut microbiota and gut colonisation by MDROs. The secondary purpose of the study is to create a biorepository of multiple body site specimens for future microbiota research.Methods and analysisThe WARRIOR project collects biological specimens, including nasal, oral and skin swabs and saliva and stool samples, along with extensive data on diet and MDRO risk factors, as an ancillary study of the Survey of the Health of Wisconsin (SHOW). The SHOW is a population-based health survey collecting data on several different health determinants and outcomes, as well as objective body measurements and biological specimens. WARRIOR participants include 600 randomly selected Wisconsin residents age 18 and over. Specimens are screened for MDRO colonisation and DNA is extracted for 16S ribosomal RNA-based microbiota sequencing. Data will be analysed to assess the relationship between dietary fibre, the gut microbiota composition and gut MDRO colonisation.Ethics and disseminationThe WARRIOR project is approved by the University of Wisconsin Institutional Review Board. The main results of this study will be published in a peer-reviewed scientific journal. |
| Databáze: | OpenAIRE |
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