Genetic analysis of mecA gene and detection of homologue pbpD in Stahylococcus sciuri group
| Autor: | Amanda C. Calazans-Silva, Irene da Silva Coelho, Shana de Mattos de Oliveira Coelho, Dayanne M. Araujo, Bruno Oliveira de Carvalho, Miliane Moreira Soares de Souza, Pedro Trivisol de Castro Medeiros |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Penicillin binding proteins
Short Communication Staphylococcus Veterinary Microbiology lcsh:QR1-502 pbpD Rodentia medicine.disease_cause Microbiology Genetic analysis lcsh:Microbiology beta-Lactam Resistance Genotype medicine Staphylococcus sciuri polycyclic compounds Animals Penicillin-Binding Proteins mecA antimicrobial resistance Horses Gene Regulator gene Oxacillin Skin Genetics biology business.industry SCCmec Ear biochemical phenomena metabolism and nutrition biology.organism_classification Anti-Bacterial Agents Nasal Mucosa Genes Bacterial business |
| Zdroj: | Brazilian Journal of Microbiology Brazilian Journal of Microbiology, Vol 45, Iss 2, Pp 651-655 (2014) Brazilian Journal of Microbiology v.45 n.2 2014 Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Volume: 45, Issue: 2, Pages: 651-655, Published: JUN 2014 |
| ISSN: | 1678-4405 1517-8382 |
| Popis: | Oxacillin/methicillin-resistance is related to the mecA and its regulatory genes mecR1 and mecI. Its origin is still unknown, although evidences support that it is related to CNS, once mecA and a homologue gene, pbpD, were both detected in Staphylococcus sciuri species group. The present work evaluated 210 samples of skin and ear swabs from rodents and 60 nasal swabs from equines of Army Biologic Institute, Rio de Janeiro. Pheno- and genotypic characterization provided 59.52% (25/42) and 78.57% (11/14) S. lentus and S. sciuri, respectively. It was observed that although all S. sciuri isolates tested positive for pbpD, there was no correlation with oxacillin-resistance. On the other hand, isolates tested positive for mecA gene also presented phenotypic oxacillin-resistance in at least one assay. The alignment of the mecA gene showed that the nucleotide sequences were sorted into 2 different groups, one comprising the bovine strains and the other containing human and equine strains. |
| Databáze: | OpenAIRE |
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