A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays
| Autor: | Gabriele Baj, Giuliana Metelli, Enrico Tongiorgi, L. Mascaretti, Ruggiero Francavilla, Marina Florean, Alessio Polacchini |
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| Přispěvatelé: | Polacchini, Alessio, Metelli, Giuliana, Francavilla, Ruggiero, Baj, Gabriele, Florean, Marina, Mascaretti, Luca Giovanni, Tongiorgi, Enrico |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Reproducibility of Result Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Article Andrology Neurotrophic factors Healthy volunteers Humans Medicine Diagnostic Overall performance Aged Whole blood Brain-derived neurotrophic factor Multidisciplinary business.industry Brain-Derived Neurotrophic Factor Reproducibility of Results Female Healthy Volunteers Middle Aged Reagent Kits Diagnostic Biomarkers Biomarker Healthy Volunteer Potential biomarkers Biomarker (medicine) Reagent Kits Sample collection business Human |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep17989 |
| Popis: | Brain-Derived Neurotrophic Factor (BDNF) has attracted increasing interest as potential biomarker to support the diagnosis or monitor the efficacy of therapies in brain disorders. Circulating BDNF can be measured in serum, plasma or whole blood. However, the use of BDNF as biomarker is limited by the poor reproducibility of results, likely due to the variety of methods used for sample collection and BDNF analysis. To overcome these limitations, using sera from 40 healthy adults, we compared the performance of five ELISA kits (Aviscera-Bioscience, Biosensis, Millipore-ChemiKineTM, Promega-Emax®, R&D-System-Quantikine®) and one multiplexing assay (Millipore-Milliplex®). All kits showed 100% sample recovery and comparable range. However, they exhibited very different inter-assay variations from 5% to 20%. Inter-assay variations were higher than those declared by the manufacturers with only one exception which also had the best overall performance. Dot-blot analysis revealed that two kits selectively recognize mature BDNF, while the others reacted with both pro-BDNF and mature BDNF. In conclusion, we identified two assays to obtain reliable measurements of human serum BDNF, suitable for future clinical applications. |
| Databáze: | OpenAIRE |
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