Quantification of VEGF isoforms and VEGFR transcripts by qRT-PCR and their significance in acute myeloid leukemia
| Autor: | Marie-Pierre Podgorniak, Géraldine Lescaille, Fabien Calvo, Raphaël Porcher, Philippe Rousselot, Hervé Dombret, Samia Mourah, Jacques Medioni, Benyoussef Naimi, Géraldine Labarchède |
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| Rok vydání: | 2009 |
| Předmět: |
0301 basic medicine
Adult Male Vascular Endothelial Growth Factor A Cancer Research medicine.medical_specialty Myeloid Angiogenesis Clinical Biochemistry Biology Sensitivity and Specificity Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine Internal medicine medicine Biomarkers Tumor Humans Protein Isoforms RNA Messenger RNA Neoplasm Receptor Aged DNA Primers Immunoassay Vascular Endothelial Growth Factor Receptor-1 Base Sequence Reverse Transcriptase Polymerase Chain Reaction Myeloid leukemia Middle Aged medicine.disease Prognosis Vascular Endothelial Growth Factor Receptor-2 Vascular endothelial growth factor Haematopoiesis Leukemia Leukemia Myeloid Acute 030104 developmental biology medicine.anatomical_structure Real-time polymerase chain reaction Endocrinology Oncology chemistry 030220 oncology & carcinogenesis Case-Control Studies Cancer research Female |
| Zdroj: | The International journal of biological markers. 24(1) |
| ISSN: | 0393-6155 |
| Popis: | Vascular endothelial growth factor (VEGF) and its receptors are known to play an important role in normal and pathological hematopoiesis but the prognostic impact of VEGF isoform transcripts in acute myeloid leukemia (AML) has not been addressed. We conducted a single-institution prospective study to analyze the impact of these angiogenic factors and the expression of their receptors on the survival of adult patients newly diagnosed with AML. We investigated the levels of VEGF transcript isoforms VEGF121, -145, -165, -189 and -206 and their receptors, VEGFR-1 and VEGFR-2, using quantitative reverse transcriptase polymerase chain reaction assays in peripheral blood mononuclear cells (PBMCs) of 67 consecutive AML patients at diagnosis. VEGF total protein was measured for comparison with mRNA levels in PBMCs. The VEGF121 splice variant transcript in AML PBMCs was significantly higher than in the normal controls. VEGF transcripts were quantified in all samples while its protein was detected in 42/67 (63%) of AML samples. High levels of VEGF121, VEGF165 transcripts and VEGF protein in AML were significantly related to a worse prognosis when analyzing overall survival (p121 expression remained an independent prognostic factor for either event-free survival or overall survival [aHR=8.83 (3.48–22.4), p121 mRNA in circulating cells from AML patients is a strong independent prognostic parameter, which could be useful in the management of unselected AML patients. |
| Databáze: | OpenAIRE |
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