Placental Biglycan Expression is Decreased in Human Idiopathic Fetal Growth Restriction
| Autor: | Paul Monagle, Fazlin Faisal, Padma Murthi, Gayathri Rajaraman, Vera Ignjatovic, Joanne M Said, Shaun P. Brennecke, Joanne L Stevenson |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male medicine.medical_specialty Decorin Placenta Placental insufficiency Biology Pathogenesis Pregnancy Fetal membrane Internal medicine Biglycan medicine Humans RNA Messenger Fetus Fetal Growth Retardation Infant Newborn Obstetrics and Gynecology medicine.disease carbohydrates (lipids) Endocrinology medicine.anatomical_structure Reproductive Medicine Case-Control Studies embryonic structures Immunohistochemistry Female Developmental Biology |
| Zdroj: | Placenta. 31:712-717 |
| ISSN: | 0143-4004 |
| DOI: | 10.1016/j.placenta.2010.05.009 |
| Popis: | Fetal growth restriction (FGR) is a leading cause of perinatal morbidity and mortality. The majority of FGR cases are idiopathic and are associated with placental insufficiency, which can result from placental thrombosis. Evidence suggests that Dermatan Sulfate (DS) is an important anti-coagulant in placentae of uncomplicated pregnancies. This study hypothesised that the expression of biglycan proteoglycan, a source of DS, is decreased in idiopathic FGR placentae compared with placentae from uncomplicated pregnancies. This study aimed to determine biglycan mRNA, protein expression and spatial distribution in idiopathic FGR placentae compared with the placentae from gestation-matched controls. Biglycan mRNA expression, protein expression and spatial distribution was determined in 26 idiopathic FGR-affected placentae and 27 placentae from gestation-matched controls (27-40 weeks gestation) using real-time PCR, immunoblotting and immunohistochemistry, respectively. Mean biglycan mRNA expression was significantly decreased in FGR placentae compared with control placentae (2.87 +/- 0.55, (n = 26) vs. 4.48 +/- 0.85, (n = 27); t-test p = 0.01). FGR placentae demonstrated a trend towards decrease in mean biglycan protein expression compared with control placentae (0.86 +/- 0.22 (n = 9, FGR) vs, 1.9 +/- 0.56 (n = 7, control) p = 0.07). Biglycan immunoreactivity was detected in endothelial cells and sub-endothelial cells of the perivascular region of fetal capillaries. Semi-quantitative analyses demonstrated a significant decrease in immunoreactive biglycan in FGR placentae compared with control placentae (51.1 +/- 19.3 vs, 500.7 +/- 223, n = 6, p < 0.001). This is the first study to demonstrate decreased biglycan expression in idiopathic FGR placentae compared to gestation-matched controls. Reduced biglycan expression may contribute to placental thrombosis within the fetal vasculature, and may contribute to the pathogenesis of idiopathic FGR. |
| Databáze: | OpenAIRE |
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