ТР53 Codon 72 Polymorphism and Human Papilloma Virus-Associated Cervical Cancer in Kyrgyz Women
| Autor: | Nazira Adasheva, E T Talaibekova, Nurbek Bukuev, Denis Vinnikov, Jainagul Isakova |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
0301 basic medicine medicine.medical_specialty Genotype cervical cancer Kyrgyz population Uterine Cervical Neoplasms Gastroenterology polymorphism law.invention Young Adult 03 medical and health sciences 0302 clinical medicine law Internal medicine medicine Humans Genetic Predisposition to Disease Allele Papillomaviridae Gene Polymerase chain reaction Aged Cervical cancer Human papilloma virus Univariate analysis Polymorphism Genetic Arg72Pro business.industry Papillomavirus Infections General Medicine Middle Aged Prognosis medicine.disease Confidence interval 030104 developmental biology ТР53 gene Case-Control Studies 030220 oncology & carcinogenesis Female Tumor Suppressor Protein p53 business Research Article Follow-Up Studies |
| Zdroj: | Asian Pacific Journal of Cancer Prevention : APJCP |
| ISSN: | 2476-762X |
| DOI: | 10.31557/apjcp.2019.20.4.1057 |
| Popis: | Background: The aim of this study was to ascertain the magnitude of association of gene ТР53 Arg72Pro polymorphic marker with cervical cancer (CC) in Kyrgyz women. Methods: We identified and included 205 women of Kyrgyz ethnicity for this case-control study, of whom N=103 were women (mean age 53.5 ± 10.0 years) with histologically confirmed CC and N=102 controls (mean age 46.5 ± 8.5 years). We detected human papilloma virus (HPV) DNA types 16 and 18 using polymerase chain reaction (PCR) with hybridization/fluorescent detection. Genotypes of ТР53 gene Arg72Pro polymorphism were identified using PCR-RFLP assay. Results: Eighty-eight percent (90/103) women with CC had HPV, of whom 43.4% (39/90) had HPV type 16, 24.4% (22/90) had HPV type 18, whereas 32.2% (29/90) carried both types. The univariate analysis of allele and genotype distribution of Arg72Pro polymorphic marker of ТР53 gene showed no difference between CC and control groups (χ2=1.24, р=0.54). However, when CC cases associated with HPV were tested against controls, Arg72 allele and Arg72Arg genotype prevalence were greater compared to controls (χ²=7.25; р=0.027 for genotypes and χ²=6.83; р=0.009 for alleles). In HPV-positive women, Arg72Arg genotype of ТР53 gene was associated with a 1.85-fold increase in the likelihood of CC (OR=1.85 [95% confidence interval (CI) 1.03-3.32]), whereas Arg72 allele increased this likelihood 1.94-fold (OR=1.94 [95% CI 1.20-3.15]). Conclusions: Arg72Arg genotype and Arg72 allele of ТР53 gene in Kyrgyz women increase the risk of HPV-associated CC. |
| Databáze: | OpenAIRE |
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