Hydrogen Sulfide Ameliorates Developmental Impairments of Rat Offspring with Prenatal Hyperhomocysteinemia

Autor: O. V. Yakovleva, Guzel F. Sitdikova, N. N. Khaertdinov, A. N. Arslanova, Svetlana Dmitrieva, Guzel Ziyatdinova, F. V. Minibayeva, Aleksey V. Yakovlev, A. R. Ziganshina, Rashid Giniatullin
Rok vydání: 2018
Předmět:
0301 basic medicine
Aging
Antioxidant
medicine.medical_treatment
Biochemistry
Antioxidants
Lipid peroxidation
chemistry.chemical_compound
0302 clinical medicine
Pregnancy
Malondialdehyde
Hydrogen Sulfide
Homocysteine
chemistry.chemical_classification
biology
Chemistry
lcsh:Cytology
Glutathione peroxidase
Brain
General Medicine
Female
Locomotion
Signal Transduction
Research Article
medicine.medical_specialty
Hyperhomocysteinemia
Article Subject
Offspring
Sulfides
Superoxide dismutase
03 medical and health sciences
Internal medicine
medicine
Animals
lcsh:QH573-671
Rats
Wistar
Glutathione Peroxidase
Superoxide Dismutase
Neurotoxicity
Cell Biology
medicine.disease
Rats
030104 developmental biology
Endocrinology
biology.protein
Lipid Peroxidation
Reactive Oxygen Species
030217 neurology & neurosurgery
Zdroj: Oxidative Medicine and Cellular Longevity
Oxidative Medicine and Cellular Longevity, Vol 2018 (2018)
ISSN: 1942-0994
Popis: Maternal high levels of the redox active amino acid homocysteine—called hyperhomocysteinemia (hHCY)—can affect the health state of the progeny. The effects of hydrogen sulfide (H2S) treatment on rats with maternal hHCY remain unknown. In the present study, we characterized the physical development, reflex ontogeny, locomotion and exploratory activity, muscle strength, motor coordination, and brain redox state of pups with maternal hHCY and tested potential beneficial action of the H2S donor—sodium hydrosulfide (NaHS)—on these parameters. Our results indicate a significant decrease in litter size and body weight of pups from dams fed with methionine-rich diet. In hHCY pups, a delay in the formation of sensory-motor reflexes was observed. Locomotor activity tested in the open field by head rearings, crossed squares, and rearings of hHCY pups at all studied ages (P8, P16, and P26) was diminished. Exploratory activity was decreased, and emotionality was higher in rats with hHCY. Prenatal hHCY resulted in reduced muscle strength and motor coordination assessed by the paw grip endurance test and rotarod test. Remarkably, administration of NaHS to pregnant rats with hHCY prevented the observed deleterious effects of high homocysteine on fetus development. In rats with prenatal hHCY, the endogenous generation of H2S brain tissues was lower compared to control and NaHS administration restored the H2S level to control values. Moreover, using redox signaling assays, we found an increased level of malondialdehyde (MDA), the end product of lipid peroxidation, and decreased activity of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GPx) in the brain tissues of rats of the hHCY group. Notably, NaHS treatment restored the level of MDA and the activity of SOD and GPx. Our data suggest that H2S has neuroprotective/antioxidant effects against homocysteine-induced neurotoxicity providing a potential strategy for the prevention of developmental impairments in newborns.
Databáze: OpenAIRE
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