Quantitative investigation of terbinafine hydrochloride absorption into a living skin equivalent model by MALDI-MSI
| Autor: | Cristina Russo, Neil Brickelbank, Catherine J. Duckett, Steve Mellor, Malcolm R. Clench, Stephen Rumbelow |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Terbinafine Hydrochloride Chromatography Antifungal Agents Chemistry Skin Absorption 010401 analytical chemistry Significant difference Absorption (skin) Mass spectrometry 01 natural sciences Mass spectrometry imaging Maldi msi 0104 chemical sciences Analytical Chemistry 03 medical and health sciences 030104 developmental biology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Skin equivalent Full thickness Isosorbide Terbinafine Skin |
| ISSN: | 1520-6882 |
| Popis: | The combination of microspotting of analytical and internal standards, matrix sublimation, and recently developed software for quantitative mass spectrometry imaging has been used to develop a high-resolution method for the determination of terbinafine hydrochloride in the epidermal region of a full thickness living skin equivalent model. A quantitative assessment of the effect of the addition of the penetration enhancer (dimethyl isosorbide (DMI)) to the delivery vehicle has also been performed, and data have been compared to those obtained from LC-MS/MS measurements of homogenates of isolated epidermal tissue. At 10% DMI, the levels of signal detected for the drug in the epidermis were 0.20 ± 0.072 mg/g tissue for QMSI and 0.28 ± 0.040 mg/g tissue for LC-MS/MS at 50% DMI 0.69 ± 0.23 mg/g tissue for QMSI and 0.66 ± 0.057 mg/g tissue for LC-MS/MS. Comparison of means and standard deviations indicates no significant difference between the values obtained by the two methods. |
| Databáze: | OpenAIRE |
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