Comparison of Hybribio GenoArray and Roche Human Papillomavirus (HPV) Linear Array for HPV Genotyping in Anal Swab Samples

Autor: Michelle I. Silver, Magaly M. Blas, Brandon Brown, Patti E. Gravitt, Huey Chi Low, Chan Yoon Leng, Yin Ling Woo
Přispěvatelé: Tang, Y-W
Rok vydání: 2015
Předmět:
Male
Microbiology (medical)
Oncology
Papillomavirus Infections/complications/virology
medicine.medical_specialty
Genotyping Techniques
Anus Neoplasms/diagnosis/virology
Anal Canal
Biology
Cervical Cancer
Microbiology
Medical and Health Sciences
Men who have sex with men
McNemar's test
Clinical Research
Virology
Internal medicine
Genotype
Genetics
medicine
Humans
Anal cancer
Clinical significance
Homosexuality
Male
Human papillomavirus
purl.org/pe-repo/ocde/ford#1.06.01 [https]
Papillomaviridae
Genotyping
Early Detection of Cancer
Cancer
Gynecology
Agricultural and Veterinary Sciences
Prevention
Papillomavirus Infections
Anal Canal/virology
Genotyping Techniques/methods
virus diseases
Homosexuality
Biological Sciences
Anus Neoplasms
medicine.disease
DNA extraction
Infectious Diseases
Papillomaviridae/classification/genetics/isolation & purification
HIV/AIDS
Sexually Transmitted Infections
Early Detection of Cancer/methods
Digestive Diseases
Zdroj: Low, HC; Silver, MI; Brown, BJ; Leng, CY; Blas, MM; Gravitt, PE; et al.(2015). Comparison of hybribio genoarray and roche human papillomavirus (HPV) linear array for HPV genotyping in anal swab samples. Journal of Clinical Microbiology, 53(2), 550-556. doi: 10.1128/JCM.02274-14. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/0484v5c1
Journal of clinical microbiology, vol 53, iss 2
ISSN: 1098-660X
0095-1137
DOI: 10.1128/jcm.02274-14
Popis: Human papillomavirus (HPV) is causally associated with anal cancer, as HPV DNA is detected in up to 90% of anal intraepithelial neoplasias and anal cancers. With the gradual increase of anal cancer rates, there is a growing need to establish reliable and clinically relevant methods to detect anal cancer precursors. In resource-limited settings, HPV DNA detection is a potentially relevant tool for anal cancer screening. Here, we evaluated the performance of the Hybribio GenoArray (GA) for genotyping HPV in anal samples, against the reference standard Roche Linear Array (LA). Anal swab samples were obtained from sexually active men who have sex with men. Following DNA extraction, each sample was genotyped using GA and LA. The overall interassay agreement, type-specific, and single and multiple genotype agreements were evaluated by kappa statistics and McNemar's χ 2 tests. Using GA and LA, 68% and 76% of samples were HPV DNA positive, respectively. There was substantial interassay agreements for the detection of all HPV genotypes (κ = 0.70, 86% agreement). Although LA was able to detect more genotypes per sample, the interassay agreement was acceptable (κ = 0.53, 63% agreement). GA had poorer specific detection of HPV genotypes 35, 42, and 51 (κ < 0.60). In conclusion, GA and LA showed good interassay agreement for the detection of most HPV genotypes in anal samples. However, the detection of HPV DNA in up to 76% of anal samples warrants further evaluation of its clinical significance.
Databáze: OpenAIRE
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