High levels of HIV-1 replication show a clear correlation with downmodulation of Bcl-2 protein in peripheral blood lymphocytes of HIV-1-seropositive subjects
| Autor: | Lucia Bertolaso, Davide Gibellini, Richard Aschbacher, M. Vignoli, Michele La Placa, Maria Carla Re, E. Ramazzotti, Giuliano Furlini |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Lymphocyte Blotting Western bel-2 Down-Regulation HIV Infections Virus Replication Epitope Virus Flow cytometry HIV-1 bel-2 apoptosis Virology Gene expression medicine Humans Lymphocytes biology medicine.diagnostic_test apoptosis virus diseases Viral Load Flow Cytometry Infectious Diseases medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Viral replication Fluorescent Antibody Technique Direct Immunology HIV-1 biology.protein Antibody Viral load |
| Zdroj: | Journal of Medical Virology. 56:66-73 |
| ISSN: | 1096-9071 0146-6615 |
| DOI: | 10.1002/(sici)1096-9071(199809)56:1<66::aid-jmv11>3.0.co;2-u |
| Popis: | Peripheral blood lymphocytes (PBLs) from 51 HIV-1-seropositive subjects with different levels of HIV-1 replication and 20 healthy blood donors were examined for the expression of the antiapoptotic Bcl-2 protein. All the plasma samples from HIV-1 patients were characterized for the presence of HIV-1 p24 and HIV, RNA viral load. Bcl-2 protein expression in fresh peripheral blood lymphocytes was studied by different tests, including Western blot and indirect immunofluorescence techniques. Direct immunofluorescence staining, revealed by flow cytometry, was applied to quantify the number of specific anti-Bcl-2 antibody epitope binding sites, thus extrapolating the relative number of Bcl-2 into the cells. The results indicate that the expression of Bcl-2 protein is significantly lower in peripheral blood lymphocytes of HIV-1-seropositive patients showing high levels of viral replication, detected by means of HIV-1 p24 and RNA viral load, with respect to HIV-1 patients with low levels of virus replication and healthy blood donors. The clear-cut inverse correlation between viral replication and Bcl-2 expression reinforces the view that HIV-1-mediated apoptosis probably represents a key mechanism in AIDS pathogenesis. |
| Databáze: | OpenAIRE |
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