Helicobacter pylori in Dyspepsia: Phenotypic and Genotypic Methods of Diagnosis
| Autor: | Ramachandra Lingadakai, Vignesh Shetty, Girisha Balaraju, Mamatha Ballal, Shiran Shetty, Ganesh C Pai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty diagnosis Biopsy 030106 microbiology Population rapid urease test Rapid urease test Chronic gastritis Gastroenterology lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine Internal medicine medicine lcsh:RC109-216 education education.field_of_study biology medicine.diagnostic_test Helicobacter pylori business.industry Phosphoglucosamine mutase phosphoglucosamine mutase gene Gold standard (test) biology.organism_classification medicine.disease culture Infectious Diseases histopathology 030211 gastroenterology & hepatology Histopathology Original Article business |
| Zdroj: | Journal of Global Infectious Diseases Journal of Global Infectious Diseases, Vol 9, Iss 4, Pp 131-134 (2017) |
| ISSN: | 0974-8245 0974-777X |
| Popis: | Background: Helicobacter pylori affects almost half of the world's population and therefore is one of the most frequent and persistent bacterial infections worldwide. H. pylori is associated with chronic gastritis, ulcer disease (gastric and duodenal), mucosa-associated lymphoid tissue lymphoma, and gastric cancer. Several diagnostic methods exist to detect infection and the option of one method or another depends on various genes, such as availability, advantages and disadvantages of each method, monetary value, and the age of patients. Materials and Methods: Patients with complaints of abdominal pain, discomfort, acidity, and loss of appetite were chosen for endoscopy, detailed history was contained, and a physical examination was conducted before endoscopy. Biopsies (antrum + body) were received from each patient and subjected to rapid urease test (RUT), histopathological examination (HPE), polymerase chain reaction (PCR), and culture. Results: Of the total 223 biopsy specimens obtained from dyspeptic patients, 122 (54.7%) were positive for H. pylori for HPE, 109 (48.9%) by RUT, 65 (29.1%) by culture, and 117 (52.5%) by PCR. The specificity and sensitivity were as follows: RUT (99% and 88.5%), phosphoglucosamine mutase PCR assay (100% and 95.9%), and culture (100% and 53.3%), respectively. Conclusion: In this study, we compared the various diagnostic methods used to identify H. pylori infection indicating that, in comparison with histology as gold standard for detection of H. pylori infection, culture and PCR showed 100% specificity whereas RUT and PCR showed 99% and 100% sensitivity, respectively. |
| Databáze: | OpenAIRE |
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