Use of various types of column reactors for flow-injection analysis
| Autor: | Masayuki Totani, Masayoshi Tabata, Jiro Endo, Takashi Murachi |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Blood Glucose
Flow injection analysis Oxidase test Chromatography L-Lactate Dehydrogenase Immobilized enzyme Chemistry Organic Chemistry Dehydrogenase General Medicine Hydrogen-Ion Concentration Enzymes Immobilized Biochemistry Reversible reaction Analytical Chemistry Enzyme catalysis Ammonia chemistry.chemical_compound Creatinine Flow Injection Analysis Humans Indicators and Reagents Creatininase |
| Zdroj: | Journal of Chromatography A. 597:435-442 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/0021-9673(92)80142-h |
| Popis: | Two or three different kinds of immobilized enzymes can be aligned in a minireactor so that sequential enzymatic reactions are carried out from upstream to downstream during flow-injection analysis. A lactate oxidase-catalase reactor, used as a precolumn for removing pre-existing lactate in serum before the lactose dehydrogenase (LDH) reaction, was useful for the determination of serum LDH activity, which did not require any blank correction. A sequential glutamate dehydrogenase-glutamate oxidase reactor was also useful for a novel chemiluminometric determination of ammonia. On the other hand, a co-immobilized creatininase-creatinase-sarcosine oxidase reactor, in spite of containing creatininase which catalyses the reversible reaction, was the most efficient for the determination of serum creatinine. |
| Databáze: | OpenAIRE |
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