Bidirectional, non-necrotizing glomerular crescents are the critical pathology in X-linked Alport syndrome mouse model harboring nonsense mutation of human COL4A5
| Autor: | Kunio Kawanishi, Michio Nagata, Kentaro Hashikami, Michiyasu Takeyama, Jiang Ying Song, Nobuyuki Saga |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Collagen Type IV Pathology medicine.medical_specialty Mutant Nonsense mutation 030232 urology & nephrology lcsh:Medicine Glomerular diseases Nephritis Hereditary urologic and male genital diseases Article Nephropathy Podocyte 03 medical and health sciences Mice 0302 clinical medicine Chronic kidney disease Glomerular Basement Membrane medicine Animals Humans Alport syndrome lcsh:Science Multidisciplinary Paediatric kidney disease business.industry urogenital system Glomerular basement membrane lcsh:R medicine.disease Actins female genital diseases and pregnancy complications Disease Models Animal Microscopy Electron 030104 developmental biology medicine.anatomical_structure Hyaluronan Receptors Codon Nonsense lcsh:Q business Nephritis Kidney disease |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | X-linked Alport syndrome (XLAS) is a progressive kidney disease caused by genetic abnormalities of COL4A5. Lack of collagen IV α5 chain staining and “basket-weave” by electron microscopy (EM) in glomerular basement membrane (GBM) are its typical pathology. However, the causal relationship between GBM defects and progressive nephropathy is unknown. We analyzed sequential pathology in a mouse model of XLAS harboring a human nonsense mutation of COL4A5. In mutant mice, nephropathy commenced from focal GBM irregularity by EM at 6 weeks of age, prior to exclusive crescents at 13 weeks of age. Low-vacuum scanning EM demonstrated substantial ragged features in GBM, and crescents were closely associated with fibrinoid exudate, despite lack of GBM break and podocyte depletion at 13 weeks of age. Crescents were derived from two sites by different cellular components. One was CD44 + cells, often with fibrinoid exudate in the urinary space, and the other was accumulation of α-SMA + cells in the thickened Bowman’s capsule. These changes finally coalesced, leading to global obliteration. In conclusion, vulnerability of glomerular and capsular barriers to the structural defect in collagen IV may cause non-necrotizing crescents via activation of PECs and migration of interstitial fibroblasts, promoting kidney disease in this model. |
| Databáze: | OpenAIRE |
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