Establishment of Three Rat Soft Tissue Tumor Cell Lines with Different Degrees of Myogenic Differentiation
| Autor: | Yoji Nagashima, K. Yu, Y. Kuchirio, S. Watanabe, Nobuyuki Yoshida, Yohei Miyagi, Kazuaki Misugi, T. Koshino, Ichiro Aoki |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Pathology
medicine.medical_specialty Mice Nude Biology Pathology and Forensic Medicine Mice Neoplasms Muscle Tissue Nude mouse Benz(a)Anthracenes Tumor Cells Cultured medicine Animals Rats Wistar Neoplasms Connective Tissue Confluency Myogenesis Muscles Mesenchymal stem cell Soft tissue Cell Differentiation Cell Biology biology.organism_classification Phenotype In vitro Rats Cell biology Cell culture Carcinogens Neoplasm Transplantation |
| Zdroj: | Pathology - Research and Practice. 190:1071-1076 |
| ISSN: | 0344-0338 |
| DOI: | 10.1016/s0344-0338(11)80903-9 |
| Popis: | Summary Soft tissue malignancies often show divergent differentiation, including myogenic lineage. Five rat tumors induced with 20-hydroxymethylcholanthrene (20-OH-MCA), were cultured in vitro, and three cell lines (YMC-1, YMC-2, YMC-3) were established from them. YMC-2 and -3 cells were spindle-shaped, and YMS-1 cells round and epithelioid. In confluency, YMC-3 cells formed myotubes. The nude mouse xenotransplants showed morphological features consistent with their myogenic phenotypes. Muscle-specific enzyme activities were highest in YMC-3 cells. These lines may be useful in the investigation of the myogenic differentiation of undifferentiated mesenchymal cells. |
| Databáze: | OpenAIRE |
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